Phosphocreatine content of freeze-clamped muscle: influence of creatine kinase inhibition.

@article{Brault2003PhosphocreatineCO,
  title={Phosphocreatine content of freeze-clamped muscle: influence of creatine kinase inhibition.},
  author={Jeffrey J Brault and Kirk A Abraham and Ronald L. Terjung},
  journal={Journal of applied physiology},
  year={2003},
  volume={94 5},
  pages={1751-6}
}
The study of cellular energetics is critically dependent on accurate measurement of high-energy phosphates. Muscle values of phosphocreatine (PCr) vary greatly between in vivo measurements (i.e., by nuclear magnetic resonance) and chemical measurements determined from muscles isolated and quick-frozen. The source of this difference has not been experimentally identified. A likely cause is activation of ATPases and phosphotransfer from PCr to ADP. Therefore, rat hindlimb skeletal muscle was… CONTINUE READING