Phosphatidylinositol 4-phosphate formation at ER exit sites regulates ER export.

  title={Phosphatidylinositol 4-phosphate formation at ER exit sites regulates ER export.},
  author={Anna Blumental-Perry and Charles J. Haney and Kelly M. Weixel and Simon C. Watkins and Ora A. Weisz and Meir Aridor},
  journal={Developmental cell},
  volume={11 5},
The mechanisms that regulate endoplasmic reticulum (ER) exit-site (ERES) assembly and COPII-mediated ER export are currently unknown. We analyzed the role of phosphatidylinositols (PtdIns) in regulating ER export. Utilizing pleckstrin homology domains and a PtdIns phosphatase to specifically sequester or reduce phosphorylated PtdIns levels, we found that PtdIns 4-phosphate (PtsIns4P) is required to promote COPII-mediated ER export. Biochemical and morphological in vitro analysis revealed… CONTINUE READING