Phosphatidylinositol 4,5-bisphosphate regulates two steps of homotypic vacuole fusion.

@article{Mayer2000Phosphatidylinositol4R,
  title={Phosphatidylinositol 4,5-bisphosphate regulates two steps of homotypic vacuole fusion.},
  author={Andreas Mayer and Dietrich Scheglmann and Stephen K. Dove and Attila Glatz and William Wickner and Albert Haas},
  journal={Molecular biology of the cell},
  year={2000},
  volume={11 3},
  pages={
          807-17
        }
}
Yeast vacuoles undergo cycles of fragmentation and fusion as part of their transmission to the daughter cell and in response to changes of nutrients and the environment. Vacuole fusion can be reconstituted in a cell free system. We now show that the vacuoles synthesize phosphoinositides during in vitro fusion. Of these phosphoinositides, phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate (PI(4,5)P(2)) are important for fusion. Monoclonal antibodies to PI(4,5)P(2… CONTINUE READING