Phosphatidylinositol-4,5-bisphosphate regulates NMDA receptor activity through alpha-actinin.

  title={Phosphatidylinositol-4,5-bisphosphate regulates NMDA receptor activity through alpha-actinin.},
  author={Ioannis E. Michailidis and Thomas D Helton and Vasileios I. Petrou and Tooraj Mirshahi and Michael D. Ehlers and Diomedes E. Logothetis},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  volume={27 20},
Phosphatidylinositol-4,5-bisphosphate (PIP2) has been shown to regulate many ion channels, transporters, and other signaling proteins, but it is not known whether it also regulates neurotransmitter-gated channels. The NMDA receptors (NMDARs) are gated by glutamate and serve as a critical control point in synaptic function. Here we demonstrate that PIP2 supports NMDAR activity. In Xenopus oocytes, overexpression of phospholipase Cgamma (PLCgamma) or preincubation with 10 microm wortmannin… CONTINUE READING