Phosphatidylethanolamine from Phosphatidylserine Decarboxylase2 is Essential for Autophagy Under Cadmium Stress in Saccharomyces cerevisiae

  title={Phosphatidylethanolamine from Phosphatidylserine Decarboxylase2 is Essential for Autophagy Under Cadmium Stress in Saccharomyces cerevisiae},
  author={Kannan Muthukumar and Vasanthi Nachiappan},
  journal={Cell Biochemistry and Biophysics},
Cadmium (Cd) is a potent toxic element used in several industries and in the process contaminates air, soil, and water. Exposure of Saccharomyces cerevisiae to Cd increases the major phospholipids, and profound increase was observed in phosphatidylethanolamine (PE). In yeast, there are four different pathways contributing to the biosynthesis of PE, and contribution to PE pool through phosphatidylserine decarboxylase2 (psd2) is not significant in normal conditions. Upon Cd exposure, psd2Δ strain… 
Phosphatidylethanolamine positively regulates autophagy and longevity
It is shown that the abundance of phosphatidylethanolamine positively regulates autophagy and that organismal life or healthspan could be positively influenced by the consumption of ethanolamine-rich food.
Lipid droplets alleviate cadmium induced cytotoxicity in Saccharomyces cerevisiae.
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Effect of cadmium on essential metals and their impact on lipid metabolism in Saccharomyces cerevisiae
The impact of Cd interference on the essential minerals such as Zn and Ca and their influence on endoplasmic reticulum stress and lipid metabolism of budding yeast is explored.
Transport of Phosphatidylserine from the Endoplasmic Reticulum to the Site of Phosphatidylserine Decarboxylase2 in Yeast
The emerging picture of the Psd2p‐specific transport pathway is one in which the enzyme and its non‐catalytic N‐terminal domains act as a hub to nucleate the assembly of a multiprotein complex, which facilitates PtdSer transport at membrane contact sites between the ER and Golgi/endosome membranes.
Autophagy Improves ARA-Rich TAG Accumulation in Mortierella alpina by Regulating Resource Allocation
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Regulation of autophagy by mitochondrial phospholipids in health and diseases.
  • Paul Hsu, Yuguang Shi
  • Biology, Medicine
    Biochimica et biophysica acta. Molecular and cell biology of lipids
  • 2017
Molecular Biology of Cadmium Toxicity in Saccharomyces cerevisiae
An attempt has been made to evaluate published cellular and molecular toxicity data related to Cd stress, and specifically published on S. cerevisiae, an eukaryotic unicellular model organism.
Cell biology, physiology and enzymology of phosphatidylserine decarboxylase.


Phosphatidylethanolamine Has an Essential Role inSaccharomyces cerevisiae That Is Independent of Its Ability to Form Hexagonal Phase Structures*
There is a critical level of PtdEtn required to support growth at elevated temperature or on the nonfermentable carbon source lactate, as well as a novel lipid determined by mass spectrometry to be phosphatidylpropanolamine (PtdPrn), which can comprise up to 40% of the total phospholipid content in supplemented cells at the expense of phosph atidylcholine and PTDEtn.
Endoplasmic reticulum is a major target of cadmium toxicity in yeast
It is shown that very low doses of Cd2+ cause ER stress in Saccharomyces cerevisiae as evidenced by the induction of the unfolded protein response (UPR) and the splicing of HAC1 mRNA, and suggests that the full functionality of the pathways involved in ER stress response is required for Cd 2+ tolerance.
Glutathione peroxidase3 of Saccharomyces cerevisiae protects phospholipids during cadmium-induced oxidative stress
Investigation of the role of glutathione peroxidase3 (gpx3) in phospholipid protection in cells found an increase in MDA over untreated, while untreated gpx3Δ cells themselves showed a higher level of MDA which was further enhanced with Cd-treatment.
Phosphatidylserine and phosphatidylethanolamine in mammalian cells: two metabolically related aminophospholipids.
  • J. Vance
  • Biology, Chemistry
    Journal of lipid research
  • 2008
Cellular levels of PS and PE are tightly regulated by the implementation of multiple compensatory mechanisms, and Elimination of either pathway is embryonically lethal, despite the normal activity of the other pathway.
Phosphatidylethanolamine Is Required for Normal Cell Morphology and Cytokinesis in the Fission Yeast Schizosaccharomyces pombe
It is demonstrated that phosphatidylethanolamine is essential for cell growth and for normal cytokinesis and cellular morphogenesis in S. pombe, and it is illustrated the usefulness of this model eukaryote for investigating potentially conserved biological and molecular functions ofosphatidylserine decarboxylamine.
Synthetic lethal interaction of the mitochondrial phosphatidylethanolamine biosynthetic machinery with the prohibitin complex of Saccharomyces cerevisiae.
Deletion of PSD1 destabilizes polypeptides encoded by the mitochondrial genome and destabilizing effects triggered by low levels of mitochondrial PtdEtn seem to account for synthetic lethality of psd1Delta with phb mutants.
Identification of a Non-mitochondrial Phosphatidylserine Decarboxylase Activity (PSD2) in the Yeast Saccharomyces cerevisiae(*)
The present studies demonstrate the presence of a second enzyme activity (denoted PSD2) which, depending on the method of evaluation, accounts for 4-12% of the total cellular phosphatidylserine decarboxylase activity found in wild type.
Cadmium-induced oxidative stress in Saccharomyces cerevisiae.
The results suggest that antioxidants were increased and are involved in the protection against macromolecular damage during oxidative stress; presumably, these enzymes are essential for counteracting the pro-oxidant effects of Cd.
Enhanced phospholipase B activity and alteration of phospholipids and neutral lipids in Saccharomyces cerevisiae exposed to N-nitrosonornicotine
It is reported for the first time that NNN exposure, significantly increases phospholipase B (PLB) activity and the preferred substrate is PC, a majorospholipid responsible for a series of metabolic functions.
Cadmium induces a heterogeneous and caspase-dependent apoptotic response in Saccharomyces cerevisiae
It is demonstrated that S. cerevisiae undergoes a glucose-dependent, programmed cell death in response to low cadmium concentrations, which is initiated within the first hour of Cd exposure, and that Cadmium-dependent apoptosis was suppressed in a gsh1Δ mutant, indicating a requirement for glutathione.