Phosphates of the naphthol AS series in the quantitative determination of alkaline and acid phosphatase activities “in situ” studied in polyacrylamide membrane model systems and by cytospectrophotometry

Abstract

Histochemical media for the demonstration of alkaline and acid phosphatases using phosphates of naphthol AS series as the substrates and various diazonium salts as the couplers were tested in the capability of reflecting various levels of enzyme activities. Polyacrylamide membranes with incorporated enzymes (various concentrations of purified enzymes as… (More)
DOI: 10.1007/BF00326609

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