Phosphate insensitive aminophosphonate mineralisation within oceanic nutrient cycles

  title={Phosphate insensitive aminophosphonate mineralisation within oceanic nutrient cycles},
  author={Jason P Chin and John P Quinn and John W. McGrath},
  journal={The ISME Journal},
Many areas of the ocean are nutrient-poor yet support large microbial populations, leading to intense competition for and recycling of nutrients. Organic phosphonates are frequently found in marine waters, but require specialist enzymes for catabolism. Previous studies have shown that the genes that encode these enzymes in marine systems are under Pho regulon control and so are repressed by inorganic phosphate. This has led to the conclusion that phosphonates are recalcitrant in much of the… 

Phosphate‐limited ocean regions select for bacterial populations enriched in the carbon–phosphorus lyase pathway for phosphonate degradation

The global scope of this analysis supports previous studies that infer phosphonate catabolism via C–P lyase is an important adaptive strategy implemented by bacteria to alleviate phosphate limitation and expands the known geographic extent and taxonomic affiliation of this metabolic pathway in the ocean.

Global and seasonal variation of marine phosphonate metabolism.

This study is the most comprehensive metagenomic survey of marine microbial phosphonate cycling to date and provides curated databases for 14 genes involved in phosphate cycling.

Aminophosphonate mineralisation is a major step in the global oceanic phosphorus redox cycle

The planktonic synthesis of reduced organophosphorus molecules, such as alkylphosphonates and aminophosphonates, represents one half of a vast global oceanic phosphorus redox cycle. Whilst

Elemental Composition, Phosphorous Uptake, and Characteristics of Growth of a SAR11 Strain in Batch and Continuous Culture

SAR11 strain HIMB114 appears to grow at efficiencies similar to those of naturally occurring bacterioplankton communities, and some of the first direct measurements of cellular elemental quotas for nitrogen (N) and phosphorus (P) for SAR11 are reported, grown in batch culture.

A widely distributed phosphate-insensitive phosphatase presents a route for rapid organophosphorus remineralization in the biosphere

Using the enzyme from Flavobacterium johnsoniae, it is shown that PafA is highly active toward phosphomonoesters, is fully functional in the presence of excess phosphate, and is essential for growth on phosphorylated carbohydrates as a sole carbon source.

A highly active phosphate-insensitive phosphatase is widely distributed in nature

It is revealed that the unique bacterial phosphatase, PafA, is a key player in the global phosphorus cycle and presents a major route for the regeneration of bioavailable phosphate required for both primary and secondary production.

Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle

The data identifies a mechanism responsible for a major oxidation process in the marine phosphorus redox cycle and suggests 2AEP may be an important source of regenerated phosphate and ammonium, which are required for oceanic primary production.

2‐Aminoethylphosphonate utilization in Pseudomonas putida  BIRD‐1 is controlled by multiple master regulators

Evidence is presented that 2AEP utilization is under dual regulation and only occurs upon depletion of C, N, or P, controlled by CbrAB, NtrBC, or PhoBR respectively, which is the first study identifying coordination between global stress response and substrate‐specific regulators in phosphonate metabolism.

Comparative transcriptomics unveil distinctive metabolic pathway of phosphonate utilization by diatom Phaeodactylum tricornutum

Phosphonates are important constituents of marine organic phosphorus, however, the bioavailability and catabolism of phosphonates by eukaryotic phytoplankton remain enigmatic. Here, we use diatom

Transcriptomic-Guided Phosphonate Utilization Analysis Unveils Evidence of Clathrin-Mediated Endocytosis and Phospholipid Synthesis in the Model Diatom, Phaeodactylum tricornutum

Phosphonates contribute ~25% of total dissolved organic phosphorus in the ocean, and are found to be important for marine phosphorus biogeochemical cycle. As a type of biogenic phosphonate produced



Phosphonate utilization by the globally important marine diazotroph Trichodesmium

The induction, by phosphorus stress, of genes from the Trichodesmium erythraeum IMS101 genome that are predicted to encode proteins associated with the high-affinity transport and hydrolysis of phosphonate compounds by a carbon–phosphorus lyase pathway are described.

The Genes and Enzymes of Phosphonate Metabolism by Bacteria, and Their Distribution in the Marine Environment

The data presented indicate the likely importance of phosphonate-P in global biogeochemical P cycling, and by extension its role in marine productivity and in carbon and nitrogen dynamics in the oceans.

Microbially mediated transformations of phosphorus in the sea: new views of an old cycle.

  • D. Karl
  • Environmental Science
    Annual review of marine science
  • 2014
The inextricable link between the P cycle and cycles of other bioelements predicts future impacts on, for example, nitrogen fixation and carbon dioxide sequestration.

Major role of planktonic phosphate reduction in the marine phosphorus redox cycle

Using simple incubation and chromatography approaches, the rate of the chemical reduction of phosphate to P(III) compounds in the western tropical North Atlantic Ocean is measured, suggesting the existence of a vast oceanic phosphorus redox cycle.

Widespread known and novel phosphonate utilization pathways in marine bacteria revealed by functional screening and metagenomic analyses.

Analysis of metagenomic databases for Pn utilization genes revealed that they are widespread and abundant among marine bacteria, suggesting that Pn metabolism is likely to play an important role in P-depleted surface waters, as well as in the more P-rich deep-water column.

Ecological nitrogen-to-phosphorus stoichiometry at station ALOHA

Screening for carbon-bound phosphorus in marine animals by high-resolution 31P-NMR spectroscopy: coastal and hydrothermal vent invertebrates.

  • L. D. QuinG. S. Quin
  • Biology
    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
  • 2001

Processes and patterns of oceanic nutrient limitation

Microbial activity is a fundamental component of oceanic nutrient cycles. Photosynthetic microbes, collectively termed phytoplankton, are responsible for the vast majority of primary production in

Microbial metabolism of reduced phosphorus compounds.

In this review, recent investigations into the biochemical pathways and molecular genetics of reduced P metabolism in bacteria are discussed and particular attention is paid to recently elucidated metabolic reactions and the genetic characterization of biosynthesis of organic reduced P compounds.


In vast regions of the ocean, a significant fraction of the vital nutrient phosphorus (P) in surface waters is associated with dissolved organic matter (DOM). Oceanic biological productivity in these