Phosphatase and oxygen radical-generating activities of mammalian purple acid phosphatase are functionally independent.

@article{Kaija2002PhosphataseAO,
  title={Phosphatase and oxygen radical-generating activities of mammalian purple acid phosphatase are functionally independent.},
  author={Helena M Kaija and Sari L. Alatalo and Jussi M. Halleen and Ylva Lindqvist and G. R. Eugenia Schneider and H. Kalervo V{\"a}{\"a}n{\"a}nen and Pirkko Vihko},
  journal={Biochemical and biophysical research communications},
  year={2002},
  volume={292 1},
  pages={128-32}
}
Bone-resorbing osteoclasts and activated macrophages express large amounts of tartrate-resistant acid phosphatase (TRAP), an iron-containing enzyme with unknown biological function. We studied acid phosphatase (AcP) and reactive oxygen species (ROS)-generating activities of recombinant rat TRAP. pH optimum was 4.5 for AcP activity and 6.5 for ROS-generating activity. Replacement of His113 and His216 by site-directed mutagenesis severely inhibited AcP activity, but had no significant effects on… CONTINUE READING

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