Phorbol ester increases the level of interleukin 2 mRNA in mitogen-stimulated human lymphocytes.

Abstract

TPA alone did not induce the production of IL 2 in human tonsillar lymphocytes but enhanced the PHA-induced IL 2 production by seven-fold. That the effect of TPA was due to an increase in IL 2 mRNA was demonstrated by examining the amount of IL 2 mRNA translatable in Xenopus laevis oocytes, and by Northern blotting analysis using IL 2 cDNA as a probe. In these ways, it was shown that TPA alone did not induce any significant IL 2 mRNA synthesis, but when added together with PHA it increased the level of IL 2 mRNA by at least 10-fold, as compared with that induced by PHA alone.

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@article{Hirano1984PhorbolEI, title={Phorbol ester increases the level of interleukin 2 mRNA in mitogen-stimulated human lymphocytes.}, author={T Hirano and Kengo Fujimoto and Tetsuo Teranishi and Naonobu Nishino and Kenji Onoue and Sakan Maeda and Kazuyuki Shimada}, journal={Journal of immunology}, year={1984}, volume={132 5}, pages={2165-7} }