Phorbol ester binding to protein kinase C requires a cysteine-rich zinc-finger-like sequence.

@article{Ono1989PhorbolEB,
  title={Phorbol ester binding to protein kinase C requires a cysteine-rich zinc-finger-like sequence.},
  author={Yoshitaka Ono and Takeshi Fujii and Kazuei Igarashi and T AKAFUMI Kuno and Chikako Tanaka and Ushio Kikkawa and Yasutomi Nishizuka},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1989},
  volume={86 13},
  pages={4868-71}
}
Protein kinase C normally has a tandem repeat of a characteristic cysteine-rich sequence in C1, the conserved region of the regulatory domain. These sequences resemble the DNA-binding zinc finger domain. For the gamma subspecies of rat brain protein kinase C, various deletion and point mutants in this domain were constructed, and the mutated proteins were expressed in Escherichia coli by using the T7 expression system. Radioactive phorbol 12,13-dibutyrate binding analysis indicated that a… CONTINUE READING
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