Phorbol ester activation of the protein kinase C pathway inhibits gonadotropin-releasing hormone gene expression.

@article{Bruder1992PhorbolEA,
  title={Phorbol ester activation of the protein kinase C pathway inhibits gonadotropin-releasing hormone gene expression.},
  author={Jan M. Bruder and W. Krebs and Terry M Nett and Margaret E. Wierman},
  journal={Endocrinology},
  year={1992},
  volume={131 6},
  pages={2552-8}
}
The effects of the phorbol ester 12-O-tetradecanoyl phorbol 13-acetate (TPA), an activator of protein kinase C (PKC), and the PKC inhibitor staurosporine on GnRH secretion and mRNA levels were studied in GT1-7 hypothalamic neuronal cells. Dose-response and time-course studies revealed that TPA (10(-8) M) acutely increased GnRH secretion 3-fold at 3-6 h, which then declined to baseline at 24 h, while it progressively decreased GnRH mRNA levels by 50% and 70% at 6 and 24 h, respectively. To… CONTINUE READING

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