Phloretinyl-3'-benzylazide: a high affinity probe for the sugar transporter in human erythrocytes. I. Hexose transport inhibition and photolabeling of mutarotase.

@article{Fannin1981Phloretinyl3benzylazideAH,
  title={Phloretinyl-3'-benzylazide: a high affinity probe for the sugar transporter in human erythrocytes. I. Hexose transport inhibition and photolabeling of mutarotase.},
  author={Franklin F. Fannin and J. O. Evans and E. Michael Gibbs and Donald F. Diedrich},
  journal={Biochimica et biophysica acta},
  year={1981},
  volume={649 2},
  pages={
          189-201
        }
}
A new phloretin derivative, phloretinyl-3'-benzylazide (PBAz), has been synthesized and compared with phloretin for its ability to inhibit the hexose transporter in human erythrocyte membranes in subdued light. Transport measurements were made using the light scattering (Orskov optical) method and a Millipore filtration technique with isotopically labeled sugars. Initial rates of sugar flux were measured under four different conditions to test for inhibition asymmetry. In each experimental… Expand
Phloretinyl-3'-benzylazide: a high affinity probe for the sugar transporter in human erythrocytes. II. Irreversible transport inhibition is induced by photolysis.
TLDR
The results reaffirm the earlier conclusions that PBAz is a potentially useful photoaffinity labeling agent for the glucose transporter in erythrocyte membranes. Expand
Asymmetrical binding of phloretin to the glucose transport system of human erythrocytes
  • R. M. Krupka
  • Chemistry, Medicine
  • The Journal of Membrane Biology
  • 2005
TLDR
The results show that the outward-facing form of the glucose carrier, but not the inward- facing form, bears a phloretin binding site; thus ph loretin, as well as cytochalasin B, is bound asymmetrically, phlorein outside and cytochardasin B inside. Expand
Reaction of the glucose carrier of erythrocytes with sodium tetrathionate: Evidence for inward-facing and outward-facing carrier conformations
  • R. M. Krupka
  • Chemistry, Medicine
  • The Journal of Membrane Biology
  • 2005
TLDR
Sodium tetrathionate reacts with the glucose carrier of human erythrocytes at a rate which is greatly altered in the presence of competitive inhibitors of glucose transport, and the properties of the binding sites are modified in unexpected ways. Expand
Photoaffinity-labeling analogs of phlorizin and phloretin: synthesis and effects on cell membranes.
  • D. Diedrich
  • Chemistry, Medicine
  • Methods in enzymology
  • 1990
TLDR
This chapter discusses the azides as membrane perturbants and photoaffinity-labeling agents and presents the results of an sodium dodecyl sulfate-polyacrylamide gel rod analysis of the radiolabeled membrane components from this experiment. Expand
Solubilization, reconstitution, and attempted affinity chromatography of the sugar transporter of the erythrocyte membrane
TLDR
Although the solubilized sugar transporter appeared to be selectively adsorbed to both PBA matrices, it could not be eluted by specific counter ligands or gentle eluants in a biologically active form, however, chaotropic agents could be used to elute intrinsic proteins, including bands 3 and 4 from the Affigel affinity medium. Expand
Photolabeling of the adipocyte hexose carrier with an aryl azide derivative of maltose
TLDR
These studies show the suitability of using carbon-1-modified sugar photoaffinity labels as probes for the hexose carrier and possibly of its regulation in rat adipocytes. Expand
Role of substrate binding forces in exchange-only transport systems: II. Implications for the mechanism of the anion exchanger of red cells
  • R. M. Krupka
  • Chemistry, Medicine
  • The Journal of Membrane Biology
  • 2005
TLDR
The transition-state theory of exchange-only membrane transport is applied to experimental results in the literature on the anion exchanger of red cells and accounts for substrate inhibition, partially noncompetitive inhibition of one substrate by another, and “tunneling”, which is net transport under conditions where exchange should prevail, according to other models. Expand
Kinetics of inhibition of transport systems.
TLDR
This chapter provides an analytical discussion of the kinetics of transport inhibition and focuses on the conventional carrier model, highlighting the importance of the carrier partition in the energized and deenergized states. Expand
Mutarotase equilibrium exchange kinetics studied by 13C-NMR.
TLDR
The rates of exchange between the alpha- and beta-anomers of D-[1-13C]glucose, at equilibrium catalyzed by porcine kidney mutarotase, were measured using 13C-NMR spin-transfer procedures and two-dimensional exchange spectroscopy. Expand
Involvement of Hexose transport in myogenic differentiation
TLDR
The fact that the glucose‐mediated increase in fusion could not be observed in Type II mutants (deficient in the HAHT transporter) suggested that myogenesis was dependent on the presence of D‐glucose or its metabolites in specific HAHT‐accessible compartments. Expand
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