Phloretinyl-3'-benzylazide: a high affinity probe for the sugar transporter in human erythrocytes. II. Irreversible transport inhibition is induced by photolysis.

@article{Fannin1982Phloretinyl3benzylazideAH,
  title={Phloretinyl-3'-benzylazide: a high affinity probe for the sugar transporter in human erythrocytes. II. Irreversible transport inhibition is induced by photolysis.},
  author={Franklin F. Fannin and J. O. Evans and Donald F. Diedrich},
  journal={Biochimica et biophysica acta},
  year={1982},
  volume={684 2},
  pages={
          228-32
        }
}
In the dark, phloretinyl-3'-benzylazide (PBAz), at a nominal concentration of 10 microM, will inhibit the transport of D-glucose in human erythrocytes by more than 90%. This inhibition can be completely reversed by percolating the cell suspension through a small column of Sephadex G-10; cells recovered after this treatment, and then loaded with 100 mM D-glucose, possess a transport capacity (glucose efflux) equal to untreated cells. The Sephadex matrix completely removes non-covalently bound… Expand
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References

SHOWING 1-9 OF 9 REFERENCES
Phloretinyl-3'-benzylazide: a high affinity probe for the sugar transporter in human erythrocytes. I. Hexose transport inhibition and photolabeling of mutarotase.
TLDR
PBAz is a potentially useful photoaffinity labeling agent capable of covalently interacting with the transporter site facing the exterior of the red cell, making it one of the most effective reversible inhibitors known. Expand
The affinity of phlorizin-like compounds for a beta-glucosidase in intestinal brush borders: comparison with the glucose transport system.
TLDR
The affinities of these compounds for the glucose transport system and phlorizin hydrolase were compared and the relative inhibitory potency of all but two of the analogs was the same in either system suggesting that there are similarities in the architecture of the two receptors. Expand
Maltosyl isothiocyanate: an affinity label for the glucose transporter of the human erythrocyte membrane. 1. Inhibition of glucose transport.
TLDR
Kinetic analysis of glucose transport after treatment of erythrocytes with MITC revealed that VT was diminished while KT was unchanged, and transportable sugars and competitive inhibitors of monosaccharide transport protected against MITC inhibition, while carbohydrates which do not interact with the transporter gave no protection. Expand
Reconstitution of D-glucose transport in vesicles composed of lipids and intrinsic protein (zone 4.5) of the human erythrocyte membrane.
TLDR
The stereospecificity and inhibition characteristics of the reconstituted transport imply that all the components of the erythrocyte D-glucose transport system are contained in the zone 4.5 membrane protein preparation. Expand
Binding of cytochalasin B to a red cell membrane protein.
  • S. Lin, J. Spudich
  • Biology, Medicine
  • Biochemical and biophysical research communications
  • 1974
TLDR
This result and other observations suggest that the sugar transport-related cytochalasin B binding sites are most likely associated with the band 3 protein. Expand
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.
TLDR
The effects of the ionic strength and pH of the hemolyzing solution on the hemoglobin content of human erythrocyte ghosts were studied in phosphate buffers and suggest an electrophysical interaction of hemoglobin with membrane constituents. Expand