Phloem unloading of potato virus X movement proteins is regulated by virus and host factors.

@article{Mekuria2008PhloemUO,
  title={Phloem unloading of potato virus X movement proteins is regulated by virus and host factors.},
  author={Tefera A. Mekuria and Devinka Bamunusinghe and Mark A Payton and Jeanmarie Verchot-Lubicz},
  journal={Molecular plant-microbe interactions : MPMI},
  year={2008},
  volume={21 8},
  pages={1106-17}
}
To determine the requirements for viral proteins exiting the phloem, transgenic plants expressing green fluorescent protein (GFP) fused to the Potato virus X (PVX) triple gene block (TGB)p1 and coat protein (CP) genes were prepared. The fused genes were transgenically expressed from the companion cell (CC)-specific Commelina yellow mottle virus (CoYMV) promoter. Transgenic plants were selected for evidence of GFP fluorescence in CC and sieve elements (SE) and proteins were determined to be… CONTINUE READING