Phleomycin Increases Transformation Efficiency and Promotes Single Integrations in Schizophyllum commune

@article{vanPeer2008PhleomycinIT,
  title={Phleomycin Increases Transformation Efficiency and Promotes Single Integrations in Schizophyllum commune},
  author={Arend van Peer and Charissa de Bekker and Arman Vinck and Han A. B. W{\"o}sten and Luis G Lugones},
  journal={Applied and Environmental Microbiology},
  year={2008},
  volume={75},
  pages={1243 - 1247}
}
ABSTRACT Phleomycin is mutagenic by introducing double-strand breaks in DNA. The ble gene of Streptoalloteychus hindustanus, which confers resistance to this substance, is widely used as a selection marker for transformation. Schizophyllum commune grows on 25 μg of phleomycin ml−1 after introduction of a resistance cassette based on the ble gene. However, we here report that growth of resistant colonies on this concentration of phleomycin resulted in aberrant colony morphologies. Apparently… 

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