Phenylmethylsulfonyl fluoride inhibits chemotactic peptide-induced actin polymerization and oxidative burst activity in human neutrophils by an effect unrelated to its anti-proteinase activity.

Stimulation of polymorphonuclear leukocytes with the chemotactic peptide N-formylmethionylleucylphenylalanine (fMet-Leu-Phe) causes conversion of monomeric actin to polymeric actin. We studied the role of proteinase inhibitors phenylmethylsulfonyl fluoride PMSF) and diisopropyl fluorophosphate in fMet-Leu-Phe-induced actin polymerization in… (More)