Phenotyping sensory nerve endings in vitro in the mouse

@article{Zimmermann2009PhenotypingSN,
  title={Phenotyping sensory nerve endings in vitro in the mouse},
  author={Katharina Zimmermann and Alexander Hein and Ulrich Hager and Jan Stefan Kaczmarek and Brian P. Turnquist and David E Clapham and Peter W Reeh},
  journal={Nature Protocols},
  year={2009},
  volume={4},
  pages={174-196}
}
This protocol details methods to identify and record from cutaneous primary afferent axons in an isolated mammalian skin–saphenous nerve preparation. The method is based on extracellular recordings of propagated action potentials from single-fiber receptive fields. Cutaneous nerve endings show graded sensitivities to various stimulus modalities that are quantified by adequate and controlled stimulation of the superfused skin with heat, cold, touch, constant punctate pressure or chemicals… CONTINUE READING
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