Phenotypic transformation of normal rat kidney cells in a growth-factor-defined medium: induction by a neuroblastoma-derived transforming growth factor independently of the EGF receptor.

@article{Zoelen1985PhenotypicTO,
  title={Phenotypic transformation of normal rat kidney cells in a growth-factor-defined medium: induction by a neuroblastoma-derived transforming growth factor independently of the EGF receptor.},
  author={Everardus J. van Zoelen and T M van Oostwaard and Paul T. van der Saag and Siegfried W. de Laat},
  journal={Journal of cellular physiology},
  year={1985},
  volume={123 2},
  pages={151-60}
}
Polypeptide growth factor activity in serum can be destroyed by treatment with dithiothreitol. When such growth-factor-inactivated serum is used as a supplement of culture media instead of regular serum, normal rat kidney (NRK) cells become quiescent unless defined polypeptide growth factors like insulin and epidermal growth factor (EGF) are added. On this basis a growth-factor-defined medium has been developed for NRK cells, which permits cell proliferation as rapidly as in media supplemented… CONTINUE READING

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