Phenotypic evaluation of cultured human mast and basophilic cells and of normal human skin mast cells

  title={Phenotypic evaluation of cultured human mast and basophilic cells and of normal human skin mast cells},
  author={Kathrin Hamann and J{\"u}rgen Grabbe and Pia Welker and Norbert Haas and Bernd Algermissen and Beate Maria Czarnetzki},
  journal={Archives of Dermatological Research},
In order to evaluate various markers for human mast cells, two human mast/basophilic cell lines (HMC-1/KU812), cultured mast cells from the peripheral blood monocytic fraction and peripheral blood monocytes were compared with mast cells in tissue sections from normal skin, using histochemistry, enzyme histochemistry and immunohistochemistry. All reagents stained normal skin mast cells, with toluidine blue, tryptase reactivity and antibodies against the FcεRI and the stem cell factor receptor (c… CONTINUE READING
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2 Excerpts

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