Phenotypic characterization of Astragalus glycyphyllos symbionts and their phylogeny based on the 16S rDNA sequences and RFLP of 16S rRNA gene

  title={Phenotypic characterization of Astragalus glycyphyllos symbionts and their phylogeny based on the 16S rDNA sequences and RFLP of 16S rRNA gene},
  author={Sebastian Gnat and Magdalena W{\'o}jcik and Sylwia Wdowiak-Wr{\'o}bel and Michał Kalita and Aneta A. Ptaszyńska and Wanda Małek},
  journal={Antonie Van Leeuwenhoek},
  pages={1033 - 1048}
In this study, the nitrogen fixing Astragalus glycyphyllos symbionts were characterized by phenotypic properties, restriction fragment length polymorphism (RFLP), and sequences of 16S rDNA. The generation time of A. glycyphyllos rhizobia in yeast extract mannitol medium was in the range 4–6 h. The studied isolates exhibited a low resistance to antibiotics, a moderate tolerance to NaCl, assimilated di- and trisaccharides, and produced acid in medium containing mannitol as a sole carbon source… 
Multilocus sequence analysis supports the taxonomic position of Astragalus glycyphyllos symbionts based on DNA-DNA hybridization.
In this study, the phylogenetic relationship and taxonomic status of six strains, representing different phenons and genomic groups of Astragalus glycyphyllos symbionts, originating from Poland, were
Characterization of Rhizobial Bacteria Nodulating Astragalus corrugatus and Hippocrepis areolata in Tunisian Arid Soils
Phenotypically, the results indicate that A. corrugatus and H. areolata isolates showed heterogenic responses to the different phenotypic features.
Phylogeny of Symbiotic Genes and the Symbiotic Properties of Rhizobia Specific to Astragalus glycyphyllos L.
The phylogeny of symbiotic genes of Astragalus glycyphyllos L. (liquorice milkvetch) nodule isolates was studied by comparative sequence analysis of nodA, nodC, nodH and nifH loci and to reflect the symbiosis-adaptive phenotype of rhizobia, it is proposed for these bacteria the new symbiovar “glycyphyLLae”, based on nodA and nodC genes sequences.
Insight into the genomic diversity and relationship of Astragalus glycyphyllos symbionts by RAPD, ERIC-PCR, and AFLP fingerprinting
The cluster analysis of RAPD, ERIC-PCR, and AFLP profiles resulted in a generally similar grouping of the test strains on generated dendrograms supporting a great potential of these DNA fingerprinting techniques for study of genomic polymorphism and evolutionary relationship of A. glycyphyllos nodulators.
Properties of Astragalus sp. microsymbionts and their putative role in plant growth promotion
The results demonstrated that the acdS gene is widespread in the genome of Astragalus sp.
Numerical analysis of phenotypic properties, genomic fingerprinting, and multilocus sequence analysis of Bradyrhizobium strains isolated from root nodules of Lembotropis nigricans of the tribe Genisteae
Bacterial strains isolated from root nodules of Lembotropis nigricans are members of Bradyrhizobium japonicum and exhibit high phenotypic and genomic diversity important for their survival in soil.
Diversity and plant growth promoting properties of rhizobia isolated from root nodules of Ononis arvensis
The studies showed that restharrow isolates formed effective symbiotic interactions with their native host (O. arvensis) and Ononis spinosa but not with Trifolium repens and Medicago sativa belonging to the same tribe TrifOLieae as Ononis species and not with Lotus corniculatus, representing the tribe Loteae.
Genetic and Phenotypic Diversity of Rhizobia Isolated from Trifolium rubens Root Nodules
The genetic and phenotypic differentiation of bacteria isolated from root nodules of Trifolium rubens, a rare clover species, and the symbiotic compatibility of selected isolates was investigated using two agronomically important plants.


Diversity of rhizobia associated with Amorpha fruticosa isolated from Chinese soils and description of Mesorhizobium amorphae sp. nov.
A new species, Mesorhizobium amorphae, is proposed for the majority of the isolates, which belonged to a moderately slow- to slow-growing, acid-producing group based upon their distinct phylogenetic position, their unique electrophoretic type, their low DNA homology, and their distinct phenotypic features.
Genetic diversity of rhizobia isolated from nodules of the relic species Vavilovia formosa (Stev.) Fed.
The studies of genetic diversity and symbiotic genes of V. formosa microsymbionts provide the primary information about their phylogeny and contribute to the conservation of this relict leguminous species.
Following phylogenetic tracks of Astragalus cicer microsymbionts
The noted congruence of the housekeeping and symbiotic gene phylogenies of A. cicer microsymbionts indicates that sym loci are transferred to these bacteria through vertical transmission without a significant participation of intergeneric horizontal gene spread.
Genista tinctoria microsymbionts from Poland are new members of Bradyrhizobium japonicum bv. genistearum.
Mesorhizobium albiziae sp. nov., a novel bacterium that nodulates Albizia kalkora in a subtropical region of China.
  • F. Wang, E. Wang, W. Chen
  • Biology
    International journal of systematic and evolutionary microbiology
  • 2007
Results indicated that the novel Mesorhizobium group associated with Albizia kalkora was a unique genomic species and was proposed as a novel species.
Phenotypic, genomic and phylogenetic characteristics of rhizobia isolated from root nodules of Robinia pseudoacacia (black locust) growing in Poland and Japan
Rhizobial strains, rescued from the root nodules of Robinia pseudoacacia growing in Japan and Poland, were characterized for the phenotypic properties, genomic diversity as well as phylogeny and
Robinia pseudoacacia in Poland and Japan is nodulated by Mesorhizobium amorphae strains
Results showed that R. pseudoacacia and M. amorphae belong to the same genomospecies, and their DNA patterns were identical or nearly identical to Mesorhizobium genus strains.
Phenotypic and genomic characteristics of rhizobia isolated from Genista tinctoria root nodules.
The relationship of G. tincoria microsymbionts to Bradyrhizobium species is suggested and the usefulness of AFLP analysis for differentiation and classification of the studied rhizobia is shown.
Genetic diversity and phylogeny of rhizobia isolated from agroforestry legume species in southern Ethiopia.
This study clearly shows that the characterization of symbionts of unexplored legumes growing in previously unexplored biogeographical areas will reveal additional diversity.
Phylogenetic analysis of rhizobia and agrobacteria based on 16S rRNA gene sequences.
The genus and species definitions of these organisms are in need of revision because they are found to be phylogenetically heterogeneous, and several subgroupings in which Rhizobium and Agrobacterium species were intermixed were evident.