Phenotypic characterization of Astragalus glycyphyllos symbionts and their phylogeny based on the 16S rDNA sequences and RFLP of 16S rRNA gene

@article{Gnat2014PhenotypicCO,
  title={Phenotypic characterization of Astragalus glycyphyllos symbionts and their phylogeny based on the 16S rDNA sequences and RFLP of 16S rRNA gene},
  author={Sebastian Gnat and Magdalena W{\'o}jcik and Sylwia Wdowiak-Wr{\'o}bel and Michał Kalita and Aneta A. Ptaszyńska and Wanda Małek},
  journal={Antonie Van Leeuwenhoek},
  year={2014},
  volume={105},
  pages={1033 - 1048}
}
In this study, the nitrogen fixing Astragalus glycyphyllos symbionts were characterized by phenotypic properties, restriction fragment length polymorphism (RFLP), and sequences of 16S rDNA. The generation time of A. glycyphyllos rhizobia in yeast extract mannitol medium was in the range 4–6 h. The studied isolates exhibited a low resistance to antibiotics, a moderate tolerance to NaCl, assimilated di- and trisaccharides, and produced acid in medium containing mannitol as a sole carbon source… 
Multilocus sequence analysis supports the taxonomic position of Astragalus glycyphyllos symbionts based on DNA-DNA hybridization.
In this study, the phylogenetic relationship and taxonomic status of six strains, representing different phenons and genomic groups of Astragalus glycyphyllos symbionts, originating from Poland, were
Characterization of Rhizobial Bacteria Nodulating Astragalus corrugatus and Hippocrepis areolata in Tunisian Arid Soils
TLDR
Phenotypically, the results indicate that A. corrugatus and H. areolata isolates showed heterogenic responses to the different phenotypic features.
Phylogeny of Symbiotic Genes and the Symbiotic Properties of Rhizobia Specific to Astragalus glycyphyllos L.
TLDR
The phylogeny of symbiotic genes of Astragalus glycyphyllos L. (liquorice milkvetch) nodule isolates was studied by comparative sequence analysis of nodA, nodC, nodH and nifH loci and to reflect the symbiosis-adaptive phenotype of rhizobia, it is proposed for these bacteria the new symbiovar “glycyphyLLae”, based on nodA and nodC genes sequences.
Insight into the genomic diversity and relationship of Astragalus glycyphyllos symbionts by RAPD, ERIC-PCR, and AFLP fingerprinting
TLDR
The cluster analysis of RAPD, ERIC-PCR, and AFLP profiles resulted in a generally similar grouping of the test strains on generated dendrograms supporting a great potential of these DNA fingerprinting techniques for study of genomic polymorphism and evolutionary relationship of A. glycyphyllos nodulators.
Properties of Astragalus sp. microsymbionts and their putative role in plant growth promotion
TLDR
The results demonstrated that the acdS gene is widespread in the genome of Astragalus sp.
Numerical analysis of phenotypic properties, genomic fingerprinting, and multilocus sequence analysis of Bradyrhizobium strains isolated from root nodules of Lembotropis nigricans of the tribe Genisteae
TLDR
Bacterial strains isolated from root nodules of Lembotropis nigricans are members of Bradyrhizobium japonicum and exhibit high phenotypic and genomic diversity important for their survival in soil.
Diversity and plant growth promoting properties of rhizobia isolated from root nodules of Ononis arvensis
TLDR
The studies showed that restharrow isolates formed effective symbiotic interactions with their native host (O. arvensis) and Ononis spinosa but not with Trifolium repens and Medicago sativa belonging to the same tribe TrifOLieae as Ononis species and not with Lotus corniculatus, representing the tribe Loteae.
Genetic and Phenotypic Diversity of Rhizobia Isolated from Trifolium rubens Root Nodules
TLDR
The genetic and phenotypic differentiation of bacteria isolated from root nodules of Trifolium rubens, a rare clover species, and the symbiotic compatibility of selected isolates was investigated using two agronomically important plants.
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