Phenotypic and genotypic evaluation of slime production by conventional and molecular microbiological techniques.

@article{Liberto2009PhenotypicAG,
  title={Phenotypic and genotypic evaluation of slime production by conventional and molecular microbiological techniques.},
  author={Maria Carla Liberto and Giovanni Matera and Angela Quirino and Angelo Giuseppe Lamberti and Renata Capicotto and Rossana Puccio and Giorgio Settimo Barreca and Emanuele Foc{\`a} and Antonio Cascio and Alfredo Foc{\`a}},
  journal={Microbiological research},
  year={2009},
  volume={164 5},
  pages={522-8}
}
Twenty-nine staphylococcal isolates from different clinical samples were tested for slime production: phenotypic characterization was carried out using Christensen test (tube test) and Congo red agar plate test (CRA plate test), while the presence and expression of icaA and icaD genes were evaluated by real-time PCR. In 79.3% of studied strains there was a concordance between slime production and presence of icaA and icaD genes, and between lack of slime production and absence of both or only… CONTINUE READING

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