Phenotypic analysis of human embryonic stem cells.

  title={Phenotypic analysis of human embryonic stem cells.},
  author={Mark D. Ungrin and Michael O'connor and Connie G Eaves and Peter W. Zandstra},
  journal={Current protocols in stem cell biology},
  volume={Chapter 1},
  pages={Unit 1B.3}
Human embryonic stem cells (hESCs) are an important tool for the study of developmental biology and may one day serve as a source of cells for regenerative medicine. As no definitive assay for hESC pluripotency is available, surrogate assays that measure markers or properties that have been correlated with hESC developmental potential are used to measure the effects of test conditions on their propagation and differentiation. This unit presents a range of protocols, including visual inspection… CONTINUE READING