Phenotypic Characterization of the Human Mast‐Cell Line HMC‐1

@article{Nilsson1994PhenotypicCO,
  title={Phenotypic Characterization of the Human Mast‐Cell Line HMC‐1},
  author={Gunnar P Nilsson and Thomas Blom and Marion Kusche-Gullberg and L Kjell{\'e}n and Joseph H. Butterfield and Christer Sundstr{\"o}m and Kenneth Nilsson and Lars Hellman},
  journal={Scandinavian Journal of Immunology},
  year={1994},
  volume={39}
}
The cell line HMC‐1, derived from a patient with mast cell leukaemia, is the only established cell line exhibiting a phenotype similar to that of human mast cells. This paper reports on a detailed characterization of the expression of a panel of markers for various types of immature and mature haematopoietic cells in the HMC‐1. We also studied the potential of HMC‐1 to differentiate upon treatment with conditioned media from the human T‐cell line Mo, retinoic acid or DMSO. 
Characterization of a Human Basophil‐Like Cell Line (LAMA‐84)
TLDR
Comparing the expression pattern of a panel of differentiation markers in LAMA‐84, and a second human cell line (KU812) expressing a basophil phenotype, it is evident that these cell lines, which presently are the only two cell lines identified with basophilic characteristics, share a large number of phenotypic characteristics.
Mast cell lines HMC‐1 and LAD2 in comparison with mature human skin mast cells – drastically reduced levels of tryptase and chymase in mast cell lines
TLDR
Mast cell lines HMC‐1 and LAD2 in comparison with mature human skin mast cells – drastically reduced levels of tryptase and chymase in mast cell lines.
Establishment and characterization of a new canine mast cell tumor cell line.
TLDR
A new cell line (CoMS) was established from a 3-year-old male mongrel dog with mast cell tumor of the oral mucosa, which will make an available source for studies on canine mast cell tumors.
Functional and phenotypic studies of two variants of a human mast cell line with a distinct set of mutations in the c‐kit proto‐oncogene
TLDR
Two sublines of HMC‐1 are described with different phenotypes and designated by the locations of specific mutations in the c‐kit proto‐oncogene, providing both information on the biological consequences of mutations in Kit and recognition of the availability of what in reality are two distinct cultured human mast cell lines.
The Fibroblast Mitogenic Activity Released from Human Basophilic Cell Line KU812 is Separate from Tryptase and PDGF Expression
TLDR
The results indicate that basophils may be potential producers of growth factors inducing fibroblast proliferation, and the factor(s) from KU812 cells stimulating fibro Blast proliferation have not been identified.
Human Mast Cell Line HMC1 Expresses Functional Mas-Related G-Protein Coupled Receptor 2
TLDR
HMC1 cells have lower levels of MRGPRX2 expression than LAD2 or HuMC, but are attractive for in vitro research because of their high growth rate and stable phenotype, and a model to render HMC1 suitable for degranulation assays by pre-incubating them with latrunculin-B (Lat-B).
The HMC-1 human mast cell line expresses the hepatocyte growth factor receptor c-met.
TLDR
Although HGF did not detectably influence the proliferation or morphology of HMC-1 cells, HGF inhibited the cells' ability to release tumor necrosis factor-alpha (TNF-alpha) in response to stimulation with PMA and the calcium ionophore, A23187.
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TLDR
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