Phenotypic Characterization of the Human Mast‐Cell Line HMC‐1

@article{Nilsson1994PhenotypicCO,
  title={Phenotypic Characterization of the Human Mast‐Cell Line HMC‐1},
  author={Gunnar P Nilsson and Thomas Blom and Marion Kusche-Gullberg and L Kjell{\'e}n and Joseph H. Butterfield and Christer Sundstr{\"o}m and Kenneth Nilsson and Lars Hellman},
  journal={Scandinavian Journal of Immunology},
  year={1994},
  volume={39}
}
The cell line HMC‐1, derived from a patient with mast cell leukaemia, is the only established cell line exhibiting a phenotype similar to that of human mast cells. This paper reports on a detailed characterization of the expression of a panel of markers for various types of immature and mature haematopoietic cells in the HMC‐1. We also studied the potential of HMC‐1 to differentiate upon treatment with conditioned media from the human T‐cell line Mo, retinoic acid or DMSO. 
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Comparing the expression pattern of a panel of differentiation markers in LAMA‐84, and a second human cell line (KU812) expressing a basophil phenotype, it is evident that these cell lines, which presently are the only two cell lines identified with basophilic characteristics, share a large number of phenotypic characteristics.
Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow
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An immortal mast cell line without Kit mutation, NCL‐2, derived from NC mouse bone marrow is established, which could be a useful tool to analyze proliferation, differentiation, and function of normal mast cells.
Mast cell lines HMC‐1 and LAD2 in comparison with mature human skin mast cells – drastically reduced levels of tryptase and chymase in mast cell lines
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Mast cell lines HMC‐1 and LAD2 in comparison with mature human skin mast cells – drastically reduced levels of tryptase and chymase in mast cell lines.
Establishment and characterization of a new canine mast cell tumor cell line.
TLDR
A new cell line (CoMS) was established from a 3-year-old male mongrel dog with mast cell tumor of the oral mucosa, which will make an available source for studies on canine mast cell tumors.
Functional and phenotypic studies of two variants of a human mast cell line with a distinct set of mutations in the c‐kit proto‐oncogene
TLDR
Two sublines of HMC‐1 are described with different phenotypes and designated by the locations of specific mutations in the c‐kit proto‐oncogene, providing both information on the biological consequences of mutations in Kit and recognition of the availability of what in reality are two distinct cultured human mast cell lines.
The Fibroblast Mitogenic Activity Released from Human Basophilic Cell Line KU812 is Separate from Tryptase and PDGF Expression
TLDR
The results indicate that basophils may be potential producers of growth factors inducing fibroblast proliferation, and the factor(s) from KU812 cells stimulating fibro Blast proliferation have not been identified.
Lectin histochemistry of human leukaemic mast cells (HMC-1) transplanted into severe combined immunodeficient (scid) mice.
TLDR
The aim of the present study is to characterise the lectin binding sites of HMC-1 cells transplanted into severe combined immunodeficient (scid) mice.
Malignant hematopoietic cell lines: in vitro models for the study of mast cell leukemia.
TLDR
Interest in this rare but nevertheless remarkable cell type should gain momentum with the availability of model systems, and the two in vitro models HMC-1 and LAD 1/2 are available.
Human Mast Cell Line HMC1 Expresses Functional Mas-Related G-Protein Coupled Receptor 2
TLDR
HMC1 cells have lower levels of MRGPRX2 expression than LAD2 or HuMC, but are attractive for in vitro research because of their high growth rate and stable phenotype, and a model to render HMC1 suitable for degranulation assays by pre-incubating them with latrunculin-B (Lat-B).
The HMC-1 human mast cell line expresses the hepatocyte growth factor receptor c-met.
TLDR
Although HGF did not detectably influence the proliferation or morphology of HMC-1 cells, HGF inhibited the cells' ability to release tumor necrosis factor-alpha (TNF-alpha) in response to stimulation with PMA and the calcium ionophore, A23187.
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References

SHOWING 1-10 OF 81 REFERENCES
Expression of a Mast Cell Tryptase in the Human Monocytic Cell Lines U‐937 and Mono Mac 6
TLDR
In the U‐937 cell line but not in normal PB monocytes, the tryptase expression was upregulated 3–50 fold following phorbol ester (PMA)‐induced differentiation, but no such induction was seen after retinoic acid, interferon‐γ or vitamin D3 exposure.
Human Leukemic Models of Myelomonocytic Development: A Review of the HL‐60 and U937 Cell Lines
The human leukemic myeloblast HL‐60 and monoblast U937 cell lines have made important contributions to the disciplines of cancer, hematology, and immunology. As sources of leukemic cells, they have…
Establishment and characterization of a human histiocytic lymphoma cell line (U‐937)
TLDR
The histiocytic origin of the cell line was shown by its capacity for lysozyme production and the strong esterase activity of the cells, and it was concluded that the U‐937 is a neoplastic, histuocytic cell line.
Establishment of an immature mast cell line from a patient with mast cell leukemia.
TLDR
A cell line showing many characteristics of immature mast cells has been established from the peripheral blood of a patient with mast cell leukemia, and solid tumors of metachromatically positive cells have been successfully induced and serially passed in nude mice using 5-azacytidine transformed cells.
Induction of Basophilic Differentiation in the Human Basophilic Cell Line KU812
TLDR
The present study has shown that the KU812 may serve as an appropriate model to study differentiation of basophils and its fast and specific response to biological factors makes it suitable as a biological assay for determination of active factor produced by atopic individuals.
Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia
TLDR
The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid differentiation and the effects of physiologic, pharmacologic, and virologic elements on this process.
Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia.
TLDR
The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid differentiation and the effects of physiologic, pharmacologic, and virologic elements on this process.
Phenotypic characterization of stem cell factor-dependent human foetal liver-derived mast cells.
TLDR
In vitro-derived mast cells derived from foetal liver in vitro expressed HLA class I, CD9, CD29, CD33, CD43, CD45 and Kit, and unlike mast cells dispersed from tissue, a high expression of CD13 was found.
Phenotypic characterization of KU812, a cell line identified as an immature human basophilic leukocyte
TLDR
A low level of expression of the eosinophil and basophil marker, major basic protein, which might indicate a relationship between basophils and eos inophil‐specific markers, is found in KU812.
Bipotential cell differentiation of KU-812: evidence of a hybrid cell line that differentiates into basophils and macrophage-like cells.
TLDR
KU-812-F belongs to the myeloid cell lineage and is at least a bipotential cell that can differentiate into basophils and macrophage-like cells, and may be a good model for basophil differentiation.
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