Phenotypic Characterization of the Human Mast‐Cell Line HMC‐1
@article{Nilsson1994PhenotypicCO, title={Phenotypic Characterization of the Human Mast‐Cell Line HMC‐1}, author={Gunnar P Nilsson and Thomas Blom and Marion Kusche-Gullberg and L Kjell{\'e}n and Joseph H. Butterfield and Christer Sundstr{\"o}m and Kenneth Nilsson and Lars Hellman}, journal={Scandinavian Journal of Immunology}, year={1994}, volume={39} }
The cell line HMC‐1, derived from a patient with mast cell leukaemia, is the only established cell line exhibiting a phenotype similar to that of human mast cells. This paper reports on a detailed characterization of the expression of a panel of markers for various types of immature and mature haematopoietic cells in the HMC‐1. We also studied the potential of HMC‐1 to differentiate upon treatment with conditioned media from the human T‐cell line Mo, retinoic acid or DMSO.
266 Citations
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HMC1 cells have lower levels of MRGPRX2 expression than LAD2 or HuMC, but are attractive for in vitro research because of their high growth rate and stable phenotype, and a model to render HMC1 suitable for degranulation assays by pre-incubating them with latrunculin-B (Lat-B).
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