Phenotypic Alteration of a Human BK ( hSlo ) Channel by hSlo b Subunit Coexpression : Changes in Blocker Sensitivity , Activation / Relaxation and Inactivation Kinetics , and Protein Kinase A Modulation

@inproceedings{Dworetzky1996PhenotypicAO,
  title={Phenotypic Alteration of a Human BK ( hSlo ) Channel by hSlo b Subunit Coexpression : Changes in Blocker Sensitivity , Activation / Relaxation and Inactivation Kinetics , and Protein Kinase A Modulation},
  author={Steven I. Dworetzky and Christopher G. Boissard and Janet T. Lum-Ragan and M C Mckay and Debra J. Post-Munson and Joanne T. Trojnacki and C Chang and Valentin K. Gribkoff},
  year={1996}
}
A human homolog of the large-conductance calcium-activated potassium (BK) channel b subunit (hSlob) was cloned, and its effects on a human BK channel (hSlo) phenotype are reported. Coexpression of hSlo and hSlob, in both oocytes and human embryonic kidney 293 cells, resulted in increased Ca sensitivity, marked slowing of BK channel activation and relaxation, and a significant reduction in slow inactivation. In addition, coexpression changed the pharmacology of the BK channel phenotype: hSlo… CONTINUE READING