Pharmacophore generation from a drug-like core molecule surrounded by a library peptide via the 10BASEd-T on bacteriophage T7.

@article{Tokunaga2014PharmacophoreGF,
  title={Pharmacophore generation from a drug-like core molecule surrounded by a library peptide via the 10BASEd-T on bacteriophage T7.},
  author={Yuuki Tokunaga and Yuuki Azetsu and Keisuke Fukunaga and Takaaki Hatanaka and Yuji Ito and Masumi Taki},
  journal={Molecules},
  year={2014},
  volume={19 2},
  pages={2481-96}
}
We have achieved site-specific conjugation of several haloacetamide derivatives into designated cysteines on bacteriophage T7-displayed peptides, which are fused to T7 capsid protein gp10. This easiest gp10 based-thioetherification (10BASEd-T) undergoes almost quantitatively like a click reaction without side reaction or loss of phage infectivity. The post-translational modification yield, as well as the site-specificity, is quantitatively analyzed by a fluorescent densitometric analysis after… CONTINUE READING