Pharmacology of muscarinic acetylcholine receptor subtypes (m1-m5): high throughput assays in mammalian cells.

@article{BrunerOsborne1996PharmacologyOM,
  title={Pharmacology of muscarinic acetylcholine receptor subtypes (m1-m5): high throughput assays in mammalian cells.},
  author={Hans Br{\"a}uner-Osborne and Mark R. Brann},
  journal={European journal of pharmacology},
  year={1996},
  volume={295 1},
  pages={93-102}
}
Based on the ability of many receptors to amplify NIH 3T3 cells, we developed a high throughput assay of cloned receptor pharmacology. In this assay, receptors are transiently co-expressed with the marker enzyme beta-galactosidase. Receptors that induce cellular proliferation select and amplify the cells that also express the marker, thus the ability of ligands to alter receptor activity are reported as changes in enzyme activity. We used this assay to evaluate the pharmacology of agonist and… CONTINUE READING