Pharmacological and biochemical characterization of purified A2a adenosine receptors in human platelet membranes by [3H]‐CGS 21680 binding

@article{Varani1996PharmacologicalAB,
  title={Pharmacological and biochemical characterization of purified A2a adenosine receptors in human platelet membranes by [3H]‐CGS 21680 binding},
  author={Katia Varani and Stefania Gessi and Alessandro Dalpiaz and Pier Andrea Borea},
  journal={British Journal of Pharmacology},
  year={1996},
  volume={117}
}
1 The binding properties of human platelet A2a adenosine receptors, assayed with the A2a‐selective agonist, [3H]‐2‐[p‐(2‐carboxyethyl)‐phenethylamino]‐5′‐N‐ethylcarboxamidoadenosine ([3H]‐CGS 21680), are masked by a non‐receptorial component, the adenotin site. In order to separate A2a receptors from adenotin sites, human platelet membranes were solubilized with 1% 3‐[(3‐cholamidopropyl)dimethylammonio]‐1‐propanesulphonate (CHAPS). The soluble platelet extract was precipitated with polyethylene… 
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