• Corpus ID: 73583328

PharmaTutor Bromelain : Applications and Purification Strategies

  title={PharmaTutor Bromelain : Applications and Purification Strategies},
  author={Ratikanta Sahoo and Pratyush Kumar Das},
Bromelain is a plant protease present in different parts of the pineapple [Ananas Comosus (Linn.) Merr.] plant. It is an enzyme of high commercial interest which can be attributed to the wide therapeutic and industrial applications it possesses. As such efficient purification and recovery of the enzyme is utmost necessary on a large scale basis. The review highlights the numerous applications of the protease enzyme and further discusses the various purification techniques beginning from simpler… 



Bromelain: an overview of industrial application and purification strategies

This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves.

Bromelain: An Overview

Bromelain is a crude extract from the fruit or stem of pineapple which consists of different closely related proteinases which are good antiinflammatory, antithrombotic and fibrinolytic agents.

Bromelain, the enzyme complex of pineapple (Ananas comosus) and its clinical application. An update.

Optimized Growth Conditions and determination of the Catalytic Type of the Peptidase Complex From a Novel Callus Culture of Pineapple (Ananas Comosus)

Catalytic behaviour, substrate-specificity and the use of a variety of inhibitors revealed its cysteine proteinase nature, suggesting it must be called callus bromelain, and the proteolytic activity showed to be higher than that obtained from the plant.

Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2.

Fruit bromelain FA2, the main proteinase component of the juice of pineapple fruit, has been purified and characterized, yielding an apparently homogeneous protein as judged by several physical, chemical, and immunochemical criteria.

Proteinase activity and stability of natural bromelain preparations.

Bioaffinity Based Oriented Immobilization of Stem Bromelain

The preparation obtained was more resistant to thermal inactivation as evident from the retention of over 50% activity after incubation at 60 for 100 min, exhibited a broader pH-activity profile with the enzyme retaining over 60% activity at pH 11 (as compared to over 25% retained by native and the enzyme immobilized covalently).

Isolation of an effective debriding agent from the stems of pineapple plants.

The stem of the pineapple plant contains, in addition to a number of proteases, particularly bromelain, a non-proteolytic component which is responsible for the complete bridement of experimental