Phantom encodes the 25-hydroxylase of Drosophila melanogaster and Bombyx mori: a P450 enzyme critical in ecdysone biosynthesis.

@article{Warren2004PhantomET,
  title={Phantom encodes the 25-hydroxylase of Drosophila melanogaster and Bombyx mori: a P450 enzyme critical in ecdysone biosynthesis.},
  author={James T. Warren and Anna Petryk and Guillermo Marqu{\'e}s and Jean-Philippe Parvy and Tetsuro Shinoda and Kyo Itoyama and Jun Kobayashi and Michael P Jarcho and Yutai Li and Michael B O'connor and Chantal Dauphin-Villemant and Lawrence I. Gilbert},
  journal={Insect biochemistry and molecular biology},
  year={2004},
  volume={34 9},
  pages={991-1010}
}
We have reported recently the identification and characterization of the last three mitochondrial cytochrome P450 enzymes (CYP) controlling the biosynthesis of 20-hydroxyecdysone, the molting hormone of insects. These are encoded by the following genes: disembodied (dib, Cyp302a1, the 22-hydroxylase); shadow (sad, Cyp315a1, the 2-hydroxylase); and shade (shd, Cyp314a1, the 20-hydroxylase). Employing similar gene identification and transfection techniques and subsequent biochemical analysis of… CONTINUE READING

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