Phallotoxins bind to actins

  title={Phallotoxins bind to actins},
  author={Theodor Wieland and Vimala Govindan},
  journal={FEBS Letters},
The function of receptors can be ascribed to the fdamentous structures which appear with increased frequency in the cytoplasma fraction of rat liver after in vivo or in vitro intoxication with phalloidin [ 11. Using [3 H] desmethylphalloin we found that 3.6 mnoles of the phallotoxin are tightly bound to 1 mg of filamentous protein [2] . Shortly thereafter it was recognized that the phallotoxin induced filaments from liver @h-filaments) bind heavy meromyosin (of rabbit muscle) in an arrow head… Expand

Topics from this paper

Interaction of phallotoxins with actin.
  • T. Wieland
  • Chemistry, Medicine
  • Advances in enzyme regulation
  • 1976
The stabilization of F-actin from rabbit muscle by PHD has been established experimentally by its behavior towards several chemical and mechanical stresses and an allosteric effect enforcing the coherence between the actin units or crosslinking by bifunctionality of the phallotoxin molecules has been postulated. Expand
Spectroscopic evidence for the interaction of phalloidin with actin
Phalloidin and secophalloidsin, a derivative which is nontoxic and does not stabilize F-actin, were samples from the authors' laboratory and Concentrations of actin and phalloids were determined spectrophotometrically. Expand
Interaction of actin with phalloidin: polymerization and stabilization of F-actin.
The cyclic peptide phalloidin, one of the toxic components of Amanita phalloides prevented the drop of viscosity of F-actin solutions after the addition of 0.6 M KI and inhibited the ATP splitting ofExpand
Trypsin protection of hepatocytes against phalloidin
The interpretation of the experimental data suggests that trypsin might induce conformational changes in the membrane structure which prevent or delay the first step of interaction between phalloidin and the membrane surface. Expand
Modification of actins by phallotoxins
  • T. Wieland
  • Biology, Medicine
  • Naturwissenschaften
  • 2004
The structure of F-actin is stabilized to such an extent that it will be resistant to the depolymerizing action of 0.6M KI and of deoxyribonuclease I, to denaturation by heat (70 °° C), and to local ruptures caused by ultrasonication or by cytochalasin B. Expand
Phalloidin counteracts the inhibitory effect of actin on deoxyribonuclease I
The toadstool poison phalloidin accelerates the polymerization of actin and stabilizes the microfilaments against 0.6 M KI, as well as against depolymerization under different conditions, e.g. by ATP, ultrasonic vibration or by cytochalasin B. Expand
Structural Effects and Functional Implications of Phalloidin and Jasplakinolide Binding to Actin Filaments.
It is shown in atomic detail that phalloidin, a mushroom toxin that is routinely used to stabilize and label actin filaments, suspends the structural changes in actin, likely influencing its interaction with actin-binding proteins. Expand
The phosphorylation of the major proteins of the human erythrocyte membrane.
  • L. Waxman
  • Biology, Medicine
  • Archives of biochemistry and biophysics
  • 1979
Both the major sialoglycoprotein (PAS-1) and the component designated by Fairbanks et al. as Band 3 are shown to be bonafide phosphoproteins by virtue of the presence of covalently bound serine and threonine phosphate residues. Expand
Structural effects and functional implications of phalloidin and jasplakinolide binding to actin filaments
It is shown in atomic detail that phalloidin, a mushroom toxin that is routinely used to stabilize and label actin filaments, suspends the structural changes in actin, likely influencing its interaction with actin binding proteins. Expand
Total Synthesis of the Death Cap Toxin Phalloidin: Atropoisomer Selectivity Explained by Molecular-Dynamics Simulations.
The enantioselective synthesis of the key amino acid (2S,4R)-4,5-dihydroxy-leucine is reported as a basis for the first total synthesis of phalloidin, which was accomplished by two different synthesis strategies. Expand


Suche nach einem Metaboliten bei Vergiftung mit Desmethylphalloin (DMP)
Various observations cited at the beginning of this paper and in the Discussion suggested that phalloidine, a poisonous constituent of the green mushroom Amanita phalloides, would not be toxic byExpand
Relation of toxicity and conformation of phallotoxins as revealed by optical rotatory dispersion studies.
Stability of the “aqueous” conformation in a lipophilic environment is apparently the second prerequisite for a toxic action of phallopeptides. Expand
Interaction of Actin and Myosin in the Absence and Presence of ATP
  • P. Dancker, M. Hoffmann
  • Chemistry, Biology
  • Zeitschrift fur Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie
  • 1973
The activation of myosin ATPase by act in-tropomyosin-troponin is reduced after tryptic digestion of actin- Troponin, which affects, according to SDS gel electro phoresis, mainly two components of troponin with molecular weights of about 32 000 and 25 000, respectively. Expand
Über die Inhaltsstoffe des grünen Knollenblätterpilzes, XXXIV1) Amanin und die Amanitine sind Sulfoxide
Aus UV-spektroskopischen Vergleichen mit zwei durch H2O2-Oxydation von Phalloidin erhaltenen Sulfoxiden (1b und 1c) und mit Skatyl-(2)-athyl-sulfoxid (2b) folgt die Sulfoxid-Natur des Amanins (7e).Expand