Phage display of cDNA libraries: enrichment of cDNA expression using open reading frame selection.

@article{Faix2004PhageDO,
  title={Phage display of cDNA libraries: enrichment of cDNA expression using open reading frame selection.},
  author={Peggy Ho Faix and Michael A. Burg and Michelle Gonzales and Edward Prenn Ravey and Andrew Baird and David Larocca},
  journal={BioTechniques},
  year={2004},
  volume={36 6},
  pages={
          1018-22, 1024, 1026-9
        }
}
Phage display technologies are powerful tools for selecting binding ligands against purified molecular targets, live cells, and organ vasculature. However, the selection of natural ligands using phage display has been limited because of significant problems associated with the display of complex cDNA repertoires. Here we describe the use of cDNA fragmentation and open reading frame (ORF) selection to display a human placental cDNA library on the pIII coat protein of filamentous phage. The… CONTINUE READING
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