Phage T4 DNA [N6-adenine]methyltransferase. Overexpression, purification, and characterization.

  title={Phage T4 DNA [N6-adenine]methyltransferase. Overexpression, purification, and characterization.},
  author={Valery G. Kossykh and Samuel L. Schlagman and Stanley Hattman},
  journal={The Journal of biological chemistry},
  volume={270 24},
The bacteriophage T4 dam gene, encoding the Dam DNA [N6-adenine]methyltransferase (MTase), has been subcloned into the plasmid expression vector, pJW2. In this construct, designated pINT4dam, transcription is from the regulatable phage lambda pR and pL promoters, arranged in tandem. A two-step purification scheme using DEAE-cellulose and phosphocellulose columns in series, followed by hydroxyapatite chromatography, was developed to purify the enzyme to near homogeneity. The yield of purified… CONTINUE READING

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