Pesticide Resistance via Transposition-Mediated Adaptive Gene Truncation in Drosophila

  title={Pesticide Resistance via Transposition-Mediated Adaptive Gene Truncation in Drosophila},
  author={Yael T. Aminetzach and J. Michael Macpherson and Dmitri A. Petrov},
  pages={764 - 767}
To study adaptation, it is essential to identify multiple adaptive mutations and to characterize their molecular, phenotypic, selective, and ecological consequences. Here we describe a genomic screen for adaptive insertions of transposable elements in Drosophila. Using a pilot application of this screen, we have identified an adaptive transposable element insertion, which truncates a gene and apparently generates a functional protein in the process. The insertion of this transposable element… 

Transposable elements contribute to the genomic response to insecticides in Drosophila melanogaster

It is found that transposable element insertions can contribute to the regulation of gene expression under insecticide exposure by rewiring cis-regulatory networks.

Resistance and the jumping gene.

This hypothesis that transposon insertion can cause resistance by altering gene product function is discussed in the light of other examples in which transposons have been implicated in insecticide resistance.

Successive Increases in the Resistance of Drosophila to Viral Infection through a Transposon Insertion Followed by a Duplication

The results demonstrate that strong selection by parasites for increased host resistance can result in major genetic changes and rapid shifts in allele frequencies; and, contrary to the prevailing view that resistance to pathogens can be a costly trait to evolve, the pleiotropic effects of these changes can have unexpected benefits.

A Transposable Element Insertion Confers Xenobiotic Resistance in Drosophila

The results provide a plausible explanation for the increase in frequency of FBti0019627 in natural populations of D. melanogaster and add to the limited number of examples in which a natural genetic mutation has been linked to its ecologically relevant phenotype.

The interplay between developmental stage and environment underlies the adaptive effect of a natural transposable element insertion

This work combines gene expression, in vivo reporter assays, CRISPR/Cas9 genome editing, and survival experiments to characterize in detail the molecular and phenotypic consequences of a natural Drosophila melanogaster transposable element insertion: the roo solo-LTR FBti0019985.

Transposable Elements Contribute to the Adaptation of Arabidopsis thaliana

The finding of expansion and contraction of different types of TEs in different A. thaliana populations highlights the importance of variations in TEs for the adaptation of plants in general in the context of rapid global climate change.

QTL mapping of natural variation reveals that the developmental regulator bruno reduces tolerance to P-element transposition in the Drosophila female germline

Genetic and cytological analyses suggest that bruno tolerance is explained by enhanced retention of germline stem cells in dysgenic ovaries, which are typically lost due to DNA damage, and implicate gametogenic regulators as a source of tolerant variants in natural populations.

The transposable element Bari‐Jheh mediates oxidative stress response in Drosophila

The results contribute to the understanding of resistance to oxidative stress in natural populations and the role of transposable elements in environmental adaptation and the replicability of fitness effects on different genetic backgrounds suggests that epistatic interactions do not seem to dominate the genetic architecture of oxidative stress resistance.

Stress response, behavior, and development are shaped by transposable element-induced mutations in Drosophila

This work analyzed the population frequencies of 1,615 Transposable Element (TE) insertions in 91 samples from 60 worldwide natural populations of Drosophila melanogaster to provide a more complete understanding of the genetic variation and the fitness-related traits relevant for adaptive evolution.



A Single P450 Allele Associated with Insecticide Resistance in Drosophila

Microarray analysis of all P450s in Drosophila melanogaster shows that DDT-R, a gene conferring resistance to DDT, is associated with overtranscription of a single cytochrome P450 gene, Cyp6g1, which has spread globally.

Strong selective sweep associated with a transposon insertion in Drosophila simulans.

  • T. SchlenkeD. Begun
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2004
We know little about several important properties of beneficial mutations, including their mutational origin, their phenotypic effects (e.g., protein structure changes vs. regulatory changes), and

Identification of a Gene Associated with Bt Resistance in Heliothis virescens

This work shows that disruption of a cadherin-superfamily gene by retrotransposon-mediated insertion was linked to high levels of resistance to the Bt toxin Cry1Ac in the cotton pest Heliothis virescens, enabling efficient DNA-based screening for resistant heterozygotes by directly detecting the recessive allele.

The population genetics of Drosophila transposable elements.

The theoretical and empirical studies reviewed here yield the conclusion that Drosophila TEs are maintained in populations as a result of transpositional increase in copy number, and that their

A test for the role of natural selection in the stabilization of transposable element copy number in a population of Drosophila melanogaster.

It is argued that a mechanism such as recombination between elements at different chromosomal sites, leading to rearrangements with highly deleterious, dominant effects could play a role in stabilizing copy number and lead to a higher abundance of elements in genomic regions with restricted crossing over.

Adaptive protein evolution at the Adh locus in Drosophila

A simple statistical test of the neutral protein evolution hypothesis is proposed based on a comparison of the number of amino-acid replacement substitutions to synonymous substitutions in the coding region of a locus, finding that there are more fixed replacement differences between species than expected.

Rapid sequence turnover at an intergenic locus in Drosophila.

No evidence suggesting that the size of this locus has been maintained over evolutionary time is found, consistent with the model of a dynamic equilibrium between persistent DNA loss through small deletions and more sporadic DNA gain through less frequent but longer insertions.

Gene Expression During the Life Cycle of Drosophila melanogaster

These studies define major characteristics of the transcriptional programs that underlie the life cycle, compare development in males and females, and show that large-scale gene expression data collected from whole animals can be used to identify genes expressed in particular tissues and organs or genes involved in specific biological and biochemical processes.

Size matters: non-LTR retrotransposable elements and ectopic recombination in Drosophila.

It is argued that TEs in these families attain high population frequencies and even reach fixation as a result of low family-wide transposition rates leading to low TE copy numbers and consequently reduced strength of selection acting on individual TE copies.