Perspectives on transgenic livestock in agriculture and biomedicine: an update.

@article{Piedrahita2011PerspectivesOT,
  title={Perspectives on transgenic livestock in agriculture and biomedicine: an update.},
  author={Jorge A. Piedrahita and Natasha J. Olby},
  journal={Reproduction, fertility, and development},
  year={2011},
  volume={23 1},
  pages={
          56-63
        }
}
It has been 30 years since the first transgenic mouse was generated and 26 years since the first example of transferring the technology to livestock was published. While there was tremendous optimism in those initial years, with most convinced that genetically modified animals would play a significant role in agricultural production, that has not come to be. So at first sight one could conclude that this technology has, to a large extent, failed. On the contrary, it is believed that it has… 
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Perspectives for feed-efficient animal production.

It is anticipated that genetically modified animals will play a significant role in shaping the future of feed-efficient and thus sustainable animal production, but will develop more slowly than the biomedical applications because of the complexity of the regulation of feed intake and metabolism.

Somatic cell nuclear transfer and transgenesis in large animals: current and future insights.

SCNT has provided a powerful tool to make copies of selected individuals in different species, to study genome pluripotency and differentiation, opening new avenues of research in regenerative medicine and representing the main route for making transgenic livestock.

Advances in the Generation of Transgenic Domestic Species via Somatic Cell Nuclear Transfer

In vitro fertilization in pigs: New molecules and protocols to consider in the forthcoming years.

Germline modification of domestic animals.

An approach currently being developed to complement SCNT for producing genetically modified animals is germ cell transplantation using genetically modified male germline stem cells (GSCs), which circumvents problems associated with embryo manipulation and nuclear reprogramming.

Xenotransplantation: Progress Along Paths Uncertain from Models to Application.

The last 2 decades have brought progressive advances in approaches that can be applied to genetic modification of large animals, which make it appropriate to consider the potential limitation of genetic engineering and of current models for advancing the clinical applications of xenotransplantation and reverse xenotranplantation.

The transformational impact of site-specific DNA modifiers on biomedicine and agriculture

CRISPR-Cas based systems in particular have broad applicability, and have low technical and economic barriers for their implementation, and will continue to have, a transformational impact in the field of gene editing in domestic animals.

Forty years of embryo transfer in cattle: a review focusing on the journal Theriogenology, the growth of the industry in North America, and personal reminisces.

Beyond the mouse monopoly: studying the male germ line in domestic animal models.

The state of the art in the isolation, characterization, culture, and manipulation of SSCs and the use of germ cell transplantation in domestic animals is focused on.

Production of Cloned Pigs with Targeted Attenuation of Gene Expression

The results demonstrate the feasibility of applying RNAi and SCNT technologies for introducing stable genetic modifications in somatic cells for eventual attenuation of gene expression in vivo in large animal species.

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