Perspectives on transgenic livestock in agriculture and biomedicine: an update.

  title={Perspectives on transgenic livestock in agriculture and biomedicine: an update.},
  author={Jorge A. Piedrahita and Natasha J. Olby},
  journal={Reproduction, fertility, and development},
  volume={23 1},
It has been 30 years since the first transgenic mouse was generated and 26 years since the first example of transferring the technology to livestock was published. While there was tremendous optimism in those initial years, with most convinced that genetically modified animals would play a significant role in agricultural production, that has not come to be. So at first sight one could conclude that this technology has, to a large extent, failed. On the contrary, it is believed that it has… 

Perspectives for feed-efficient animal production.

It is anticipated that genetically modified animals will play a significant role in shaping the future of feed-efficient and thus sustainable animal production, but will develop more slowly than the biomedical applications because of the complexity of the regulation of feed intake and metabolism.

Somatic cell nuclear transfer and transgenesis in large animals: current and future insights.

SCNT has provided a powerful tool to make copies of selected individuals in different species, to study genome pluripotency and differentiation, opening new avenues of research in regenerative medicine and representing the main route for making transgenic livestock.

Germline modification of domestic animals.

An approach currently being developed to complement SCNT for producing genetically modified animals is germ cell transplantation using genetically modified male germline stem cells (GSCs), which circumvents problems associated with embryo manipulation and nuclear reprogramming.

Xenotransplantation: Progress Along Paths Uncertain from Models to Application.

The last 2 decades have brought progressive advances in approaches that can be applied to genetic modification of large animals, which make it appropriate to consider the potential limitation of genetic engineering and of current models for advancing the clinical applications of xenotransplantation and reverse xenotranplantation.

The transformational impact of site-specific DNA modifiers on biomedicine and agriculture

CRISPR-Cas based systems in particular have broad applicability, and have low technical and economic barriers for their implementation, and will continue to have, a transformational impact in the field of gene editing in domestic animals.

Beyond the mouse monopoly: studying the male germ line in domestic animal models.

The state of the art in the isolation, characterization, culture, and manipulation of SSCs and the use of germ cell transplantation in domestic animals is focused on.

Production of Cloned Pigs with Targeted Attenuation of Gene Expression

The results demonstrate the feasibility of applying RNAi and SCNT technologies for introducing stable genetic modifications in somatic cells for eventual attenuation of gene expression in vivo in large animal species.



Making transgenic livestock: Genetic engineering on a large scale

The full potential of the transgenic livestock system will not be fully realized until: (1) gene constructs can be designed that function in a reproducible, predictable manner; and (2) the genetic control of physiological processes are more clearly elucidated.

Transgenic pigs as models for translational biomedical research

An overview of the current techniques for genetic modification of pigs and the transgenic pig models established for neurodegenerative diseases, cardiovascular diseases, cystic fibrosis, and diabetes mellitus is provided.

Transgenic farm animals: an update.

The first transgenic livestock species were reported in 1985. Since then microinjection of foreign DNA into pronuclei of zygotes has been the method of choice. It is now being replaced by more

No shortcuts to pig embryonic stem cells.

Advances in the generation of transgenic pigs via embryo-derived and primordial germ cell-derived cells.

This work isolated and cultured pig primordial germ cells (PGC) while maintaining them in an undifferentiated state as determined by morphology and alkaline phosphatase (AP) activity and identified two chimaeric fetuses that contain tissues with the transgene incorporated into their chromosomes.

Production of gene-targeted sheep by nuclear transfer from cultured somatic cells

Efficient and reproducible gene targeting in fetal fibroblasts to place a therapeutic transgene at the ovine α1(I) procollagen (COL1A1) locus is described and the production of live sheep by nuclear transfer is described.

Gene targeting from laboratory to livestock: Current status and emerging concepts

The current state of the art in the field of cell‐mediated transgenesis is reviewed, the crucial aspects of the methodology are discussed and an overview of emerging approaches to increase the efficiency of gene targeting in somatic cells are provided.

Recent progress in embryonic stem cell research and its application in domestic species.

Embryonic germ cells may represent such an alternative as they showed a higher plasticity than ESCs as contributed to embryonic development forming chimeric newborns but, as for ESCs, standardization is still far away and efficiency is very low.

Cattle Mammary Bioreactor Generated by a Novel Procedure of Transgenic Cloning for Large-Scale Production of Functional Human Lactoferrin

The results indicate that co-microinjection with a BAC and a marker gene into donor cells for somatic cell cloning indeed improves transgenic efficiency, and the cattle mammary bioreactors generated with this novel procedure produce functional rhLF on an industrial scale.

Transgenic-cloned pigs systemically expressing red fluorescent protein, Kusabira-Orange.

It is demonstrated that transgenic-cloned pigs exhibiting systemic red fluorescence expression can be efficiently produced by nuclear transfer of somatic cells retrovirally transduced with huKO gene.