Perspectives on transgenic livestock in agriculture and biomedicine: an update.
@article{Piedrahita2011PerspectivesOT, title={Perspectives on transgenic livestock in agriculture and biomedicine: an update.}, author={Jorge A. Piedrahita and Natasha J. Olby}, journal={Reproduction, fertility, and development}, year={2011}, volume={23 1}, pages={ 56-63 } }
It has been 30 years since the first transgenic mouse was generated and 26 years since the first example of transferring the technology to livestock was published. While there was tremendous optimism in those initial years, with most convinced that genetically modified animals would play a significant role in agricultural production, that has not come to be. So at first sight one could conclude that this technology has, to a large extent, failed. On the contrary, it is believed that it has…
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References
SHOWING 1-10 OF 90 REFERENCES
Making transgenic livestock: Genetic engineering on a large scale
- BiologyJournal of cellular biochemistry
- 1992
The full potential of the transgenic livestock system will not be fully realized until: (1) gene constructs can be designed that function in a reproducible, predictable manner; and (2) the genetic control of physiological processes are more clearly elucidated.
Transgenic pigs as models for translational biomedical research
- BiologyJournal of Molecular Medicine
- 2010
An overview of the current techniques for genetic modification of pigs and the transgenic pig models established for neurodegenerative diseases, cardiovascular diseases, cystic fibrosis, and diabetes mellitus is provided.
Transgenic farm animals: an update.
- BiologyReproduction, fertility, and development
- 2007
The first transgenic livestock species were reported in 1985. Since then microinjection of foreign DNA into pronuclei of zygotes has been the method of choice. It is now being replaced by more…
Advances in the generation of transgenic pigs via embryo-derived and primordial germ cell-derived cells.
- BiologyJournal of reproduction and fertility. Supplement
- 1997
This work isolated and cultured pig primordial germ cells (PGC) while maintaining them in an undifferentiated state as determined by morphology and alkaline phosphatase (AP) activity and identified two chimaeric fetuses that contain tissues with the transgene incorporated into their chromosomes.
Production of gene-targeted sheep by nuclear transfer from cultured somatic cells
- BiologyNature
- 2000
Efficient and reproducible gene targeting in fetal fibroblasts to place a therapeutic transgene at the ovine α1(I) procollagen (COL1A1) locus is described and the production of live sheep by nuclear transfer is described.
Gene targeting from laboratory to livestock: Current status and emerging concepts
- BiologyBiotechnology journal
- 2009
The current state of the art in the field of cell‐mediated transgenesis is reviewed, the crucial aspects of the methodology are discussed and an overview of emerging approaches to increase the efficiency of gene targeting in somatic cells are provided.
Recent progress in embryonic stem cell research and its application in domestic species.
- BiologyReproduction in domestic animals = Zuchthygiene
- 2008
Embryonic germ cells may represent such an alternative as they showed a higher plasticity than ESCs as contributed to embryonic development forming chimeric newborns but, as for ESCs, standardization is still far away and efficiency is very low.
Cattle Mammary Bioreactor Generated by a Novel Procedure of Transgenic Cloning for Large-Scale Production of Functional Human Lactoferrin
- Biology, EngineeringPloS one
- 2008
The results indicate that co-microinjection with a BAC and a marker gene into donor cells for somatic cell cloning indeed improves transgenic efficiency, and the cattle mammary bioreactors generated with this novel procedure produce functional rhLF on an industrial scale.
Transgenic-cloned pigs systemically expressing red fluorescent protein, Kusabira-Orange.
- BiologyCloning and stem cells
- 2008
It is demonstrated that transgenic-cloned pigs exhibiting systemic red fluorescence expression can be efficiently produced by nuclear transfer of somatic cells retrovirally transduced with huKO gene.