Perspectives on the in vitro culture of filariae

  title={Perspectives on the in vitro culture of filariae},
  author={Mary S. Cupp},
  journal={In Vitro Cellular & Developmental Biology - Animal},
A primary constraint in the culture of human filariae is in obtaining starting material—either microfilariae (mfs), which infect invertebrates, or third stage larvae (L3s), which are infective to humans. Cryopreservation methods which partially overcome this difficulty have been developed for both mfs and L3s. Complete development of mfs to L3s outside an intact host was obtained recently when mosquito thoraces infected byBrugia malayi (24 h after the bloodmeal) were maintained in vitro. In… CONTINUE READING