Perspectives on Anti-Glycan Antibodies Gleaned from Development of a Community Resource Database

@article{Sterner2016PerspectivesOA,
  title={Perspectives on Anti-Glycan Antibodies Gleaned from Development of a Community Resource Database},
  author={Eric Sterner and Natalie Flanagan and Jeffrey C. Gildersleeve},
  journal={ACS Chemical Biology},
  year={2016},
  volume={11},
  pages={1773 - 1783}
}
Antibodies are used extensively for a wide range of basic research and clinical applications. While an abundant and diverse collection of antibodies to protein antigens have been developed, good monoclonal antibodies to carbohydrates are much less common. Moreover, it can be difficult to determine if a particular antibody has the appropriate specificity, which antibody is best suited for a given application, and where to obtain that antibody. Herein, we provide an overview of the current state… 

Figures and Tables from this paper

A combined computational-experimental approach to define the structural origin of antibody recognition of sialyl-Tn, a tumor-associated carbohydrate antigen
TLDR
A generalizable approach employing high-throughput techniques for characterizing the structure and specificity of anti-carbohydrate monoclonal antibodies developed against the tumor-associated carbohydrate antigen sialyl-Tn (STn), which would allow rational design of potent antibodies targeting carbohydrates.
Efficient Screening of Glycan-Specific Aptamers Using a Glycosylated Peptide as a Scaffold.
TLDR
This approach obviated a tedious glycan preparation process and allowed favorable expose of the intrinsic flexible conformation of natural glycans, which holds great promise for developing glycan-specific aptamers for challenging applications such as cancer targeting.
Anti-glycan antibodies: roles in human disease.
TLDR
This review aims to highlight some of the well-studied and critically important applications of anti-carbohydrate antibodies.
Generation of Novel Anti-MUC1 Monoclonal Antibodies with Designed Carbohydrate Specificities Using MUC1 Glycopeptide Library
TLDR
This study established two novel anti-MUC1 monoclonal antibodies (1B2 and 12D10) with designed carbohydrate specificities that showed a strong binding to not only native MUC1 but also 20-mer glycopeptide with a monovalent epitope and should become powerful tools for biological studies on M UC1 O-glycan structures.
Selective Recognition of Carbohydrate Antigens by Germline Antibodies Isolated from AID Knockout Mice.
TLDR
A distinct approach for isolating germline antibodies that involves immunizing activation-induced cytidine deaminase (AID) knockout mice is presented and insights into the structural basis of glycan recognition are provided.
Presentation Mode of Glycans Affect Recognition of Human Serum anti-Neu5Gc IgG Antibodies.
TLDR
The results demonstrate that the presentation mode and diversity of Neu5Gc-glycans are critical for detection of the full collection of human serum anti-Neu5gc IgGs, which can cause exacerbation of diseases mediated by chronic inflammation such as cancer and atherosclerosis.
Emergence and significance of carbohydrate-specific antibodies
TLDR
This work focuses on the significance of the intestinal microbiota in shaping carbohydrate-specific antibodies not just in the gut, but also in the blood circulation, and addresses the definition of natural antibodies and the production of carbohydrate- specific antibodies upon antigen stimulation.
Biomolecular recognition of the glycan neoantigen CA19-9 by distinct antibodies
TLDR
This work analyzes the biomolecular recognition of a single tumor-associated carbohydrate antigen CA19-9 by two distinct antibodies using X-ray crystallography and reveals strategies used by antibodies to specifically recognize glycan antigens and shows how automated antibody-optimization methods may be used to enhance the clinical potential of existing antibodies.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 103 REFERENCES
High-throughput carbohydrate microarray profiling of 27 antibodies demonstrates widespread specificity problems.
TLDR
More than half of the antibodies considered to be specific for their designated antigen were found to cross-react with other glycans, suggesting that cross-reactive glycans could be mistaken for the designated antigen in biopsy samples or other biological samples, leading to inaccurate conclusions.
Multidimensional glycan arrays for enhanced antibody profiling.
TLDR
Neoglycoprotein density is a useful element of diversity for evaluating antibody recognition and, when combined with variations in glycan structure and glycan density, provides multidimensional glycan arrays with enhanced performance for monoclonal antibody development, biomarker discovery, and vaccine optimization.
Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens.
TLDR
A protocol to generate highly affine monoclonal antibodies against pure and defined synthetic carbohydrate antigens of high specificity, selectivity, and affinity that may be useful for a variety of biochemical and medical applications is described.
Diverse molecular recognition properties of blood group A binding monoclonal antibodies.
TLDR
Eight monoclonal antibodies to the blood group A (BG-A) antigen were evaluated to assess specificity, relative affinity and the influence of glycan density on recognition, providing new insights into the recognition properties of anti- BG-A antibodies.
Cross-platform comparison of glycan microarray formats.
TLDR
A new method was developed to facilitate systematic comparisons across distinct array platforms and the strongest binders of each lectin were identified using the universal threshold across all platforms while identification of weaker binders was influenced by platform-specific factors including presentation of determinants, array composition and self-reported thresholding methods.
An essential epitope of anti-MUC1 monoclonal antibody KL-6 revealed by focused glycopeptide library.
Human serum Krebs von den Lungen-6 (KL-6) antigen, a high-molecular-weight glycoprotein classified as a polymorphic epithelial mucin (MUC1), is a biomarker of diseases such as interstitial pneumonia,
Human Monoclonal Antibodies to Sialyl-Lewisa (CA19.9) with Potent CDC, ADCC, and Antitumor Activity
TLDR
On the basis of the potential of sLea as a target for immune attack and their affinity, specificity, and effector functions, 5B1and 7E3 may have clinical utility.
A systematic approach to protein glycosylation analysis: a path through the maze.
TLDR
Some of the major technologies routinely used for structural N- and O-glycan analysis are introduced, describing the complementary information that each provides.
The repertoire of glycan determinants in the human glycome.
TLDR
Based on the current knowledge of the composition of the glycome and the size of GBP binding sites, glycoproteins and glycolipids may contain approximately 3000 glycan determinants with an additional approximately 4000 theoretical pentasaccharide sequences in glycosaminoglycans, which provide an achievable target for new chemical and/or enzymatic syntheses.
Cancer biomarkers defined by autoantibody signatures to aberrant O-glycopeptide epitopes.
TLDR
This work detected cancer-associated IgG autoantibodies in sera from breast, ovarian, and prostate cancer patients against different aberrent O-glycopeptide epitopes derived from MUC1, representing a previously unaddressed source of sensitive biomarkers for early detection of cancer.
...
1
2
3
4
5
...