Persistent activation of GABA(A) receptor/Cl(-) channels by astrocyte-derived GABA in cultured embryonic rat hippocampal neurons.

@article{Liu2000PersistentAO,
  title={Persistent activation of GABA(A) receptor/Cl(-) channels by astrocyte-derived GABA in cultured embryonic rat hippocampal neurons.},
  author={Q. Y. Liu and Anne E Schaffner and Yoong Hee Chang and Dragan Maric and Jeffery L. Barker},
  journal={Journal of neurophysiology},
  year={2000},
  volume={84 3},
  pages={1392-403}
}
Whole cell patch-clamp recordings using Cl(-)-filled pipettes revealed more negative levels of baseline current and associated current variance in embryonic rat hippocampal neurons co-cultured on a monolayer of astrocytes than those cultured on poly-D-lysine. These effects were mimicked by culturing neurons on poly-D-lysine in astrocyte-conditioned medium (ACM). The baseline current and variance decreased immediately in all cells after either local perfusion with saline or exposure to… CONTINUE READING