Peroxynitrite production by TNF-alpha and IL-1beta: implication for suppression of osteoblastic differentiation.

@article{Hikiji2000PeroxynitritePB,
  title={Peroxynitrite production by TNF-alpha and IL-1beta: implication for suppression of osteoblastic differentiation.},
  author={Hisako Hikiji and Wee Soo Shin and Toshiyuki Koizumi and Tsuyoshi Takato and Takafumi Susami and Yoko Koizumi and YOKO OKAI-MATSUO and Teruhiko Toyo-Oka},
  journal={American journal of physiology. Endocrinology and metabolism},
  year={2000},
  volume={278 6},
  pages={E1031-7}
}
To determine the roles of nitric oxide (NO) and its metabolite, peroxynitrite (ONOO(-)), on osteoblastic activation, we investigated the effects of a NO donor [ethanamine, 2, 2'-(hydroxynitrosohydrazono)bis- (dNO)], an O(-2) donor (pyrogallol), and an ONOO(-) scavenger (urate) on alkaline phosphatase (ALPase) activity and osteocalcin gene expression, which are indexes of osteoblastic differentiation. dNO elevated ALPase activity in the osteogenic MC3T3-E1 cell line. The combination of dNO and… CONTINUE READING