Peroxynitrite inhibits leukocyte-endothelial cell interactions and protects against ischemia-reperfusion injury in rats.

@article{Lefer1997PeroxynitriteIL,
  title={Peroxynitrite inhibits leukocyte-endothelial cell interactions and protects against ischemia-reperfusion injury in rats.},
  author={D. Lefer and R. Scalia and B. Campbell and T. Nossuli and R. Hayward and M. Salamon and J. Grayson and A. Lefer},
  journal={The Journal of clinical investigation},
  year={1997},
  volume={99 4},
  pages={
          684-91
        }
}
Peroxynitrite (ONOO-) anion, formed by the interaction of superoxide with nitric oxide (NO), has previously been implicated as a cytotoxic agent. However, the effects of this free radical species on neutrophil (PMN)-endothelial cell interactions is largely unknown. We investigated the direct actions of ONOO- on PMN adhesion to endothelial cells in vitro and in vivo, as well as the effects of ONOO- on PMN-mediated myocardial ischemia-reperfusion injury. In vitro, peroxynitrite (100-1,000 nM… Expand
Peroxynitrite is not a major mediator of endothelial cell injury by activated neutrophils in vitro.
TLDR
No-derived ONOO- is not a major cytotoxic mediator in BAEC injury by activated PMN, and NO may have a cytoprotective effect by inhibiting PMN adherence to endothelial cells. Expand
Mechanisms of cardioprotection by peroxynitrite in myocardial ischemia and reperfusion injury.
TLDR
Only maximally achievable concentrations of ONOO- exert significant cardioprotective effects, in part by decreasing surface expression of P-selectin and decreasing PMN-endothelium interactions in the rat mesentery. Expand
Peroxynitrite reduces myocardial infarct size and preserves coronary endothelium after ischemia and reperfusion in cats.
TLDR
It is suggested that physiologically relevant concentrations of ONOO- exert significant cardioprotective and vasculoprotective effects in MI/R in cats, at least partially by attenuating PMN-endothelium interactions. Expand
Effects of nitric oxide and peroxynitrite on endotoxin‐induced leukocyte adhesion to endothelium
TLDR
Data demonstrating inhibition of leukocyte adhesion to endotoxin‐treated endothelium suggest that peroxynitrite or NO may exert a beneficial effect during sepsis. Expand
Crucial role of local peroxynitrite formation in neutrophil-induced endothelial cell activation.
TLDR
Endothelial cells adjacent to neutrophils may have elevated levels of peroxynitrite that result in an increased expression of adhesion molecules that represent a preferential site for adhesion and migration of additional neutrophs when simultaneously high concentrations of NO and neutrophil-derived superoxide are present. Expand
Role of Nitric Oxide in Myocardial Ischemia-Reperfusion Injury
TLDR
Amplification of endogenously generated NO with the precursor L-arginine and increased tissue levels with parenteral administration of various classes of NO donor agents have been used to effectively reduce neutrophil-endothelium interactions, the accumulation of neutrophils in reperfused myocardium, endothelial dysfunction, and infarction. Expand
Nitric oxide-dependent and independent effects on human platelets treated with peroxynitrite.
TLDR
Peroxynitrite exerts dual effects on platelets, which are either activating or inhibitory due to the conversion of ONOO- to NO or NO donors, and it is hypothesized that the conversion to NO is the critical factor determining the outcome of OnOO- exposure in vivo. Expand
Inhibition of nitric oxide synthase attenuates peroxynitrite generation, but augments neutrophil accumulation in hepatic ischemia-reperfusion in rats.
TLDR
The results suggest that NO is important in attenuating neutrophil accumulation and liver damage in ischemia-reperfusion injury and suggests that the anti-inflammatory function of NO is more important than the cytotoxic potential of peroxynitrite in acute inflammation. Expand
Peroxynitrite, a two-edged sword in post-ischemic myocardial injury-dichotomy of action in crystalloid- versus blood-perfused hearts.
TLDR
Results demonstrate that ONOO(-) may act as a "double-edged sword" in postischemic myocardial injury, which is directly toxic to the cardiac tissue at a relatively high concentration, but it can indirectly protect myocardials cells from neutrophil-induced injury at a much lower concentration. Expand
Inhibition of Nitric Oxide Synthase Attenuates Peroxynitrite Generation , but Augments Neutrophil Accumulation in Hepatic Ischemia-Reperfusion in Rats 1
The role of nitric oxide (NO) and peroxynitrite in the process of neutrophil adhesion and infiltration was investigated in a model of hepatic ischemia-reperfusion. Male Fischer rats were subjected toExpand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 52 REFERENCES
Nitric oxide attenuates leukocyte-endothelial interaction via P-selectin in splanchnic ischemia-reperfusion.
TLDR
It is concluded that the effects of exogenous NO on leukocyte-endothelial interaction after ischemia-reperfusion appear to be at least partially mediated through the endothelial adhesion molecule P-selectin. Expand
Involvement of nitric oxide and nitrosothiols in relaxation of pulmonary arteries to peroxynitrite.
TLDR
ONOO- appears to relax vascular tissue, in part, by nitrosylating tissue GSH (or other thiols), which subsequently releases NO over prolonged time periods. Expand
Paradoxical fate and biological action of peroxynitrite on human platelets.
TLDR
The fate and therefore the actions of ONOO- in biological systems are critically dependent on the biological environment in which this oxidant is present. Expand
Inhibition of endothelial-derived nitric oxide promotes P-selectin expression and actions in the rat microcirculation.
TLDR
Data indicate an important functional relationship between endothelial-derived NO production and expression of the endothelial adhesion molecule P-selectin. Expand
Coronary Vascular and Antiplatelet Effects of Peroxynitrite in Human Tissues
The present study investigated the interrelationship between nitric oxide and its intermediate degradation product, peroxynitrite, on reactivity of isolated human coronary arteries and washed humanExpand
Peroxynitrite-induced Accumulation of Cyclic GMP in Endothelial Cells and Stimulation of Purified Soluble Guanylyl Cyclase
TLDR
The data suggest that the biologic activity of ONOO involves S-nitrosation of cellular thiols resulting in NO-mediated cyclic GMP accumulation. Expand
Reactive species in ischemic rat lung injury: contribution of peroxynitrite.
TLDR
The levels of the stable decomposition products of nitric oxide and peroxynitrite, nitrite plus nitrate, were twofold greater than control during reperfusion after 60 min of ischemia, and binding of an anti-nitrotyrosine antibody was observed in reperfused lungs. Expand
Inhibition of nitric oxide production. Mechanisms of vascular albumin leakage.
TLDR
The findings suggest that the increased albumin leakage observed in postcapillary venules after inhibition of nitric oxide production involves a mechanism that includes a role for cGMP, platelet activating factor, leukocyte-endothelial cell adhesion, and the endothelial cell cytoskeleton. Expand
In vivo neutralization of P-selectin protects feline heart and endothelium in myocardial ischemia and reperfusion injury.
TLDR
It is demonstrated that PMN adherence to endothelium by P-selectin is an important early consequence of reperfusion injury, and a specific monoclonal antibody to P- selectin exerts significant endothelial preservation and cardioprotection in myocardial ischemia and reperfusions. Expand
Peroxynitrite induces both vasodilatation and impaired vascular relaxation in the isolated perfused rat heart.
TLDR
A wide range of concentrations of ONOO- induce a vascular dysfunction, as evidenced by the tachyphylaxis to its own vasodilator actions and the long-lasting impairment of the responses to other vasodilant responses, which at certain concentrations of OnOO- is sufficient to counteract its deleterious action. Expand
...
1
2
3
4
5
...