Peroxynitrite induces destruction of the tetrahydrobiopterin and heme in endothelial nitric oxide synthase: transition from reversible to irreversible enzyme inhibition.

@article{Chen2010PeroxynitriteID,
  title={Peroxynitrite induces destruction of the tetrahydrobiopterin and heme in endothelial nitric oxide synthase: transition from reversible to irreversible enzyme inhibition.},
  author={Weiguo Chen and Lawrence J. Druhan and Chun-An Chen and Craig F. Hemann and Yeong-Renn Chen and Vladim{\'i}r Berka and Ah-lim Tsai and Jay L. Zweier},
  journal={Biochemistry},
  year={2010},
  volume={49 14},
  pages={3129-37}
}
Endothelial nitric oxide synthase (eNOS) is an important regulator of vascular and cardiac function. Peroxynitrite (ONOO(-)) inactivates eNOS, but questions remain regarding the mechanisms of this process. It has been reported that inactivation is due to oxidation of the eNOS zinc-thiolate cluster, rather than the cofactor tetrahydrobiopterin (BH(4)); however, this remains highly controversial. Therefore, we investigated the mechanisms of ONOO(-)-induced eNOS dysfunction and their dose… CONTINUE READING