Peroxynitrite-induced apoptosis in photoreceptor cells.

  title={Peroxynitrite-induced apoptosis in photoreceptor cells.},
  author={Shinji Ito and G. S. Wu and Takashi Kimoto and Toshio Hisatomi and T Ishibashi and Narsing Adupa Rao},
  journal={Current eye research},
  volume={28 1},
PURPOSE To study the mechanisms of peroxynitrite-induced photoreceptor cell damage, using retinal cultures and a peroxynitrite donor, 3-morpholinosydnonimine (SIN-1). METHODS Retinal explants obtained from 20-day-old Lewis rat pups, were exposed to SIN-1 for varying lengths of time at varying concentrations. Apoptosis in the photoreceptor cells was detected using the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method and a DNA fragmentation assay. Selected retinal samples were… CONTINUE READING