Peroxynitrite inactivation of human cytochrome P450s 2B6 and 2E1: heme modification and site-specific nitrotyrosine formation.

@article{Lin2007PeroxynitriteIO,
  title={Peroxynitrite inactivation of human cytochrome P450s 2B6 and 2E1: heme modification and site-specific nitrotyrosine formation.},
  author={Hsia-lien Lin and Eugene Myshkin and Lucy A Waskell and Paul F. Hollenberg},
  journal={Chemical research in toxicology},
  year={2007},
  volume={20 11},
  pages={1612-22}
}
This study examined the reaction of peroxynitrite (PN) with two human cytochrome P450s, P450 2B6 (2B6) and P450 2E1 (2E1). After the reaction with PN, the NADPH/reductase-supported 7-ethoxy-4-(trifluoromethyl)coumarin (EFC) deethylation activity of both P450s was decreased in a concentration-dependent manner. HPLC analysis revealed that the prosthetic heme group of 2B6 was modified but to a lesser extent than the decrease in enzymatic activity. In contrast, the heme moiety of 2E1 was not… CONTINUE READING

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