Peroxisome dynamics in cultured mammalian cells.

  title={Peroxisome dynamics in cultured mammalian cells.},
  author={Sofie J Huybrechts and Paul P Van Veldhoven and Chantal Brees and Guy P. Mannaerts and Georgyi V. Los and Marc Fransen},
  volume={10 11},
Despite the identification and characterization of various proteins that are essential for peroxisome biogenesis, the origin and the turnover of peroxisomes are still unresolved critical issues. In this study, we used the HaloTag technology as a new approach to examine peroxisome dynamics in cultured mammalian cells. This technology is based on the formation of a covalent bond between the HaloTag protein--a mutated bacterial dehalogenase which is fused to the protein of interest--and a… CONTINUE READING

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