Peroxisome Degradation by Microautophagy in Pichia pastoris: Identification of Specific Steps and Morphological Intermediates

@article{Sakai1998PeroxisomeDB,
  title={Peroxisome Degradation by Microautophagy in Pichia pastoris: Identification of Specific Steps and Morphological Intermediates },
  author={Yasuyoshi Sakai and Antonius A. Koller and Linda K. Rangell and Gilbert A. Keller and Suresh Subramani},
  journal={The Journal of Cell Biology},
  year={1998},
  volume={141},
  pages={625 - 636}
}
We used the dye N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenylhexatrienyl ) pyridinium dibromide (FM4-64) and a fusion protein, consisting of the green fluorescent protein appended to the peroxisomal targeting signal, Ser-Lys-Leu (SKL), to label the vacuolar membrane and the peroxisomal matrix, respectively, in living Pichia pastoris cells and followed by fluorescence microscopy the morphological and kinetic intermediates in the vacuolar degradation of peroxisomes by microautophagy and… CONTINUE READING

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