Peroxisomal β‐oxidation of polyunsaturated fatty acids in Saccharomyces cerevisiae: isocitrate dehydrogenase provides NADPH for reduction of double bonds at even positions

@article{Roermund1998PeroxisomalO,
  title={Peroxisomal β‐oxidation of polyunsaturated fatty acids in Saccharomyces cerevisiae: isocitrate dehydrogenase provides NADPH for reduction of double bonds at even positions},
  author={C. V. van Roermund and E. Hettema and A. J. Kal and M. van den Berg and H. Tabak and R. Wanders},
  journal={The EMBO Journal},
  year={1998},
  volume={17}
}
  • C. V. van Roermund, E. Hettema, +3 authors R. Wanders
  • Published 1998
  • Biology, Medicine
  • The EMBO Journal
  • The β‐oxidation of saturated fatty acids in Saccharomyces cerevisiae is confined exclusively to the peroxisomal compartment of the cell. Processing of mono‐ and polyunsaturated fatty acids with the double bond at an even position requires, in addition to the basic β‐oxidation machinery, the contribution of the NADPH‐dependent enzyme 2,4‐dienoyl‐CoA reductase. Here we show by biochemical cell fractionation studies that this enzyme is a typical constituent of peroxisomes. As a consequence, the… CONTINUE READING
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    References

    SHOWING 1-10 OF 61 REFERENCES
    The membrane of peroxisomes in Saccharomyces cerevisiae is impermeable to NAD(H) and acetyl‐CoA under in vivo conditions.
    • 310
    • Highly Influential
    Metabolic aspects of peroxisomal β-oxidation
    • 253
    Peroxisomes and beta-oxidation of long-chain unsaturated carboxylic acids.
    • J. Hiltunen
    • Medicine
    • Scandinavian journal of clinical and laboratory investigation. Supplementum
    • 1991
    • 4
    Peroxisomal beta-oxidation. Purification of four novel 3-hydroxyacyl-CoA dehydrogenases from rat liver peroxisomes.
    • 62
    [Peroxisomal beta-oxidation].
    • 19
    beta-Oxidation in Candida tropicalis. Partial purification and biological function of an inducible 2,4-dienoyl coenzyme A reductase.
    • 44
    • Highly Influential
    Pip2p: a transcriptional regulator of peroxisome proliferation in the yeast Saccharomyces cerevisiae.
    • 109