Peroxidative modification of a membrane protein. Conformation-dependent chemical modification of adenine nucleotide translocase in Cu2+/tert-butyl hydroperoxide treated mitochondria.

@article{GirnCalle1996PeroxidativeMO,
  title={Peroxidative modification of a membrane protein. Conformation-dependent chemical modification of adenine nucleotide translocase in Cu2+/tert-butyl hydroperoxide treated mitochondria.},
  author={Julio Gir{\'o}n-Calle and Harald H. O. Schmid},
  journal={Biochemistry},
  year={1996},
  volume={35 48},
  pages={15440-6}
}
Peroxidative treatment of rat heart mitochondria results in a gradual increase of the apparent molecular weight of the adenine nucleotide translocase (ANT) by up to 1.2 kDa. ANT isolated from mitochondria treated with 1 mM tert-butyl hydroperoxide and 5-40 microM Cu2+ for 1 h at 37 degrees C exhibited a progressive loss of lysine, cysteine, arginine, and valine residues compared to native ANT. N-Ethylmaleimide, dithiothreitol, and the specific inhibitor of ANT, carboxyatractyloside (CAT… CONTINUE READING