Permeation of the luminal capillary glycocalyx is determined by hyaluronan.

@article{Henry1999PermeationOT,
  title={Permeation of the luminal capillary glycocalyx is determined by hyaluronan.},
  author={C. Henry and B. Duling},
  journal={The American journal of physiology},
  year={1999},
  volume={277 2},
  pages={
          H508-14
        }
}
The endothelial cell glycocalyx influences blood flow and presents a selective barrier to movement of macromolecules from plasma to the endothelial surface. In the hamster cremaster microcirculation, FITC-labeled Dextran 70 and larger molecules are excluded from a region extending almost 0.5 micrometer from the endothelial surface into the lumen. Red blood cells under normal flow conditions are excluded from a region extending even farther into the lumen. Examination of cultured endothelial… Expand
Shedding of the endothelial glycocalyx in arterioles, capillaries, and venules and its effect on capillary hemodynamics during inflammation.
TLDR
Results indicate that fMLP caused shedding of glycans in all microvessels with reductions in thickness of the barrier to 70-kDa dextran of 110, 80, and 123 nm, in arterioles, capillaries, and venules, respectively. Expand
Localization of the permeability barrier to solutes in isolated arteries by confocal microscopy.
TLDR
The presence of a thick ESL that contributes to the permeability barrier to solutes is demonstrated, which is sensitive to phototoxic stress, and its damage could form an early event in atherosclerosis. Expand
EFFECT OF HYALURONIDASE TREATMENT ON THE STRUCTURAL INTEGRITY OF THE ENDOTHELIAL GLYCOCALYX LAYER
EFFECT OF HYALURONIDASE TREATMENT ON THE STRUCTURAL INTEGRITY OF THE ENDOTHELIAL GLYCOCALYX LAYER By Kristin LeAnne Simmons A thesis submitted in partial fulfillment of the requirements for theExpand
Fluorescence correlation spectroscopy can probe albumin dynamics inside lung endothelial glycocalyx.
TLDR
The utility of fluorescence correlation spectroscopy (FCS) to measure albumin diffusion rates and concentration profiles above the cell surface and overlying the intercellular junctions of lung capillary endothelial cells is demonstrated and Hyaluronidase digestion completely eliminated albumin-glycocalyx interactions. Expand
Endothelial Glycocalyx: Permeability Barrier and Mechanosensor
TLDR
It is concluded that many of the barrier properties of the whole surface layer are not representative of the primary fiber matrix forming the molecular filter determining transvascular exchange. Expand
ROLE OF DIVALENT CATIONS IN GLYCOCALYX SHEDDING AND LEUKOCYTE-ENDOTHELIUM ADHESION
  • 2008
The endothelial cell surface layer, also known as the glycocalyx, is composed of macromolecules including glycoproteins and proteoglycans. The proteoglycan network consists of glycosaminoglycans suchExpand
Composition of the endothelial glycocalyx and its relation to its thickness and diffusion of small solutes.
TLDR
The fMLP-induced shedding caused a reduction in glycocalyx thickness and as with heparinase, decreased the diffusion coefficient of solutes (which may decrease permeability), which suggests that the removal of heparan sulfate may cause a collapse of the glycocalyX which counters decreases in thickness by compacting the layer to maintain a constant resistance to filtration. Expand
Effect of acute hyaluronidase treatment of the glycocalyx on tracer-based whole body vascular volume estimates in mice.
TLDR
It is indicated that the glycocalyx contributes to size-dependent differences in whole body vascular distribution of plasma solutes in mice, and Whole body vascular volume measurements based on the differential distribution of glycocalyX-selective tracers appear appropriate for the detection of generalized glyocalyx degradation in experimental animals and humans. Expand
Glycocalyx and endothelial (dys) function: from mice to men.
TLDR
Glycocalyx disruption is accompanied by enhanced sensitivity of the vasculature towards atherogenic stimuli which emphasizes that not only the composition of the glycocalyx is important in facilitating these properties but that the contribution of its physical dimension and barrier properties should also be considered. Expand
Inflammation- and ischemia-induced shedding of venular glycocalyx.
TLDR
The composition of the glycocalyx results from a balance of the rate of biosynthesis of GAGs by the endothelial cell and their shedding, which may be mediated by intracellular and/or membrane-bound proteases or lyases released or activated by G protein signaling. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 30 REFERENCES
Identification of distinct luminal domains for macromolecules, erythrocytes, and leukocytes within mammalian capillaries.
TLDR
It is concluded that the wall of skeletal muscle capillaries is decorated with a 0.4- to 0.5-microns-thick endothelial surface coat, which may represent the true active interface between blood and the capillary wall. Expand
Plasma proteins modify the endothelial cell glycocalyx of frog mesenteric microvessels.
TLDR
The interaction of plasma proteins with the endothelial cell using cationized ferritin as a marker of the cell surface glycocalyx is investigated using single microvessels of the frog mesentery to assess the effects of these proteins on the structure and function of the cells. Expand
Heparinase treatment suggests a role for the endothelial cell glycocalyx in regulation of capillary hematocrit.
TLDR
Hparinase treatment results in a sustained elevation in the capillary tube hematocrit and a dissociation of the typical relationship between vasomotor changes and red cell distribution in capillaries, suggesting that physiological stimuli which alter the number of red blood cells within capillary may operate by modifying interactions between plasma and one or more components on the luminal surface of capillary. Expand
Perturbation of the blood-retinal barrier after enzyme perfusion. A cytochemical study.
  • R. M. Pino
  • Biology, Medicine
  • Laboratory investigation; a journal of technical methods and pathology
  • 1987
TLDR
Findings indicate that a cell-surface heparan sulfate proteoglycan (or a nonidentified protein removed by proteolysis) is a key molecule required for the maintenance of the blood-retinal barrier. Expand
Differential lectin binding to microvascular endothelial glycoconjugates during normal angiogenesis in the chick chorioallantoic membrane.
TLDR
Analysis of temporal and spatial lectin binding to carbohydrate moieties of luminal microvascular endothelia of the chick embryo chorioallantoic membrane suggests a possible association between expression of endothelial glycoconjugates and the ontogeny of microv vascular perm-selectivity during normal angiogenesis. Expand
Permeability of frog mesenteric capillaries after partial pronase digestion of the endothelial glycocalyx.
  • R. Adamson
  • Biology, Medicine
  • The Journal of physiology
  • 1990
TLDR
The moderate increases in Lp induced by pronase perfusion are associated with partial digestion of the endothelial glycocalyx but are not accompanied by changes in the dimensions of the intercellular cleft, supporting the fibre matrix hypothesis of capillary permeability and suggesting that the endothelium contributes as much as 60% of the hydraulic resistance of the capillary wall. Expand
The dynamic structure of the pericellular matrix on living cells
TLDR
It is reported that the chondrocyte pericellular matrix is a dynamic structure consisting of individual tethered molecular complexes which project outward from the cell surface which undergo restricted rotation or wobbling. Expand
The ultrastructural basis of endothelial cell surface functions.
TLDR
The surface replication technique, coupled with immunocytochemical procedures, facilitates studies of the topographies of surface enzymes such as angiotensin converting enzyme (ACE) and carboxypeptidase N (CPN), and provides unique views of the glycocalyx, a cell coating previously believed to be amorphous but now seen to be a highly organized carpet-work under which surface enzymes, receptors, etc are embedded. Expand
Hyaluronan and its binding proteins, the hyaladherins.
  • B. Toole
  • Biology, Medicine
  • Current opinion in cell biology
  • 1990
TLDR
Hyaluronan is a high-molecular-weight, highly anionic polysacchande found in extracellular matrices and at ceU surfaces and its unique physicochemical properties have fascinated polymer biophysicists and physiologists for decades. Expand
Association of hyaluronan with rat vascular endothelial and smooth muscle cells.
  • P. Eggli, W. Graber
  • Chemistry, Medicine
  • The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
  • 1995
TLDR
The intra- and extracellular aspects of vascular endothelial and smooth muscle cell plasma membranes revealed distinct labeling, with a high affinity for caveolae being manifested in both cell types and in all kinds of vessels. Expand
...
1
2
3
...