Permeation of NH3/NH4 + and cell pH in colonic crypts of the rat

  title={Permeation of NH3/NH4 + and cell pH in colonic crypts of the rat},
  author={Mar{\'i}a Vald{\'e}s Ram{\'i}rez and Ricardo Fernandez and Gerhard Malnic},
  journal={Pfl{\"u}gers Archiv},
Colon cells are subjected to high concentrations of NH3 and NH4 +, and a sizeable portion of this buffer is absorbed. The flux of these components into cells causes opposite effects on their pH; this effect is largely used to induce an acid load and to observe the mechanism of acid extrusion from cells. We studied cells of microdissected colon crypts loaded with BCECF and superfused with NH4Cl-containing Krebs-Ringer solution. We found a marked transient reduction in pH measured by ratiometric… CONTINUE READING
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Regulation of ion transport in colonic crypts

R Greger, M Bleich, J Leipziger, D Ecke, M Mall
News Physiol Sci • 1997

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