Permeabilization of cell membranes.

@article{Jamur2010PermeabilizationOC,
  title={Permeabilization of cell membranes.},
  author={Maria C{\'e}lia Jamur and Constance Oliver},
  journal={Methods in molecular biology},
  year={2010},
  volume={588},
  pages={
          63-6
        }
}
In order to detect intracellular antigens, cells must first be permeabilized especially after fixation with cross-linking agents such as formaldehyde and glutaraldehyde. Permeabilization provides access to intracellular or intraorganellar antigens. Two general types of reagents are commonly used: organic solvents, such as methanol and acetone, and detergents such as saponin, Triton X-100 and Tween-20. The organic solvents dissolve lipids from cell membranes making them permeable to antibodies… CONTINUE READING
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