Permeabilization and lysis of Pseudomonas pseudoalcaligenes cells by Triton X-100 for efficient production of d-malate

@article{Werf2004PermeabilizationAL,
  title={Permeabilization and lysis of Pseudomonas pseudoalcaligenes cells by Triton X-100 for efficient production of d-malate},
  author={Mari{\"e}t J. van der Werf and Sybe Hartmans and Will J. J. van den Tweel},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={43},
  pages={590-594}
}
Pseudomonas pseudoalcaligenes can only form d-malate from maleate after incubation of the cells with a solvent or a detergent. The effect of the detergent Triton X-100 on d-malate production was studied in more detail. The longer the cells were incubated with Triton X-100, the higher was the d-malate production activity, until the maximal malease activity… CONTINUE READING